Cell dispensing device and cell dispensing method
AssigneeSinfonia Technology Co., Ltd.
InventorsDaichi Horii, Hiroyuki Uda, Hideki Maeda, Haruki Takeuchi
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Abstract
A cell dispensing device capable of efficiently dispensing cells while reducing the risk of mixing of bacteria or the like is provided. The cell dispensing device includes a first tube configured to connect a container and a dispensing container to each other, a syringe pump attached to a second tube connected to a branch portion formed in the first tube, and a buffer tank provided between the syringe pump and the branch portion. The cell suspension stored in the container is temporarily stored in the buffer tank and is delivered to the dispensing container by the syringe pump.
Claims6
A cell dispensing method for dispensing cells, while maintaining an aseptic state of a cell dispensing device, by using the cell dispensing device that includes a container configured to store a cell suspension, at least one dispensing container configured to store the cell suspension dispensed from the container, a connection path including a first connection tube configured to connect the container and the at least one dispensing container to each other and a second connection tube connected to a first branch portion formed in the first connection tube, a pump part installed to the second connection tube, a buffer part arranged between the first branch portion and the pump part, and a first degassing part configured to have one end connected to the second connection tube and another end opened to atmosphere, the method comprising:
- a degassing step of sucking a gas inside the at least one dispensing container by the pump part to remove the gas inside the at least one dispensing container and discharging the gas inside the pump part to the atmosphere through the first degassing part;
- a suction step of sucking the cell suspension from the container by the pump part and storing the cell suspension in the buffer part;
- and
- a dispensing step of delivering a part or an entire of the cell suspension stored in the buffer part to the at least one dispensing container by the pump part.
Dependencies are inferred from the claim text.