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US 12,383,655 B2Granted
Methods of isolating and using descemet's membrane and compositions including isolated descemet's membrane
AssigneeRegents of the University of Minnesota
InventorsJoshua Honghan Hou, Peter Bedard, Ching Yuan
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Abstract
This disclosure describes methods of preparing a decellularized Descemet's membrane and an isolated Descemet's membrane, methods of using an isolated Descemet's membrane, and tissues prepared using an isolated Descemet's membrane. This disclosure further describes a composition that includes an isolated Descemet's membrane. In some embodiments, the tissues and methods described herein may be used to treat a limbal stem cell deficiency or as an ocular surface bandage.
Claims25
A method comprising:
- removing endothelium from a Descemet's membrane of a cornea to provide a decellularized Descemet's membrane;
- separating the decellularized Descemet's membrane from the stroma of the cornea to obtain an isolated Descemet's membrane;
- adding epithelial cells to the isolated Descemet's membrane in vitro, wherein the epithelial cells are corneal epithelial cells or limbal epithelial cells;
- and
- culturing the epithelial cells in culture medium that promotes proliferation of the epithelial cells on the anterior surface of the isolated Descemet's membrane.
2 more elements
A method comprising:
- removing endothelium from a Descemet's membrane of a cornea to provide a decellularized Descemet's membrane;
- separating the decellularized Descemet's membrane from the stroma of the cornea to obtain an isolated Descemet's membrane;
- and
- preparing the isolated Descemet's membrane for transplantation to a subject;
- and
- transplanting the isolated Descemet's membrane to an ocular surface of a patient in need thereof, wherein the patient exhibits at least one of a partial limbal stem cell deficiency, a total limbal stem cell deficiency, an ocular surface trauma, a persistent epithelial defect, an epithelial erosion, a recurrent erosion, a corneal ulcer, a corneal melt, or an ocular surface disease.
3 more elements
Dependencies are inferred from the claim text.