Single domain antibody targeting αVβ3 integrin
AssigneeKorea Basic Science Institute
InventorsKi-Yuk Chang, Kwan-Soo Hong, Chan-Woo Kim, Hyeon-Seung Lee, Hyun-Min Kim
Life of this patent
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Abstract
Disclosed are an αvβ3 integrin targeting single-domain antibody and various applications thereof. The αvβ3 integrin targeting single-domain antibody exhibits high binding ability to αvβ3 integrin related to angiogenesis, excellent tissue permeability, and biostability compared to conventional antibodies. Further, the single-domain antibody may be combined with fluorescent particles and thus may be easily measured in vitro, in vivo or ex vivo, and may be effective in detecting angiogenesis and diagnosing angiogenesis related diseases, therefore it may be usefully used in related industries.
Claims15
An αvβ3 integrin targeting single-domain antibody encoded by at least one selected from a group consisting of base sequences of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, and SEQ ID NO:
- 10.
A recombinant vector containing at least one selected from a group consisting of base sequences represented by SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, and SEQ ID NO:
- 10.
A method for preparing an αvβ3 integrin targeting single-domain antibody, the method comprising:
- (1) culturing a recombinant microorganism transformed with a recombinant vector containing at least one selected from a group consisting of base sequences represented by SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7 SEQ ID NO:
- 8, SEQ ID NO:
- 9 and SEQ ID NO:
- 10;
- and
- (2) expressing a single-domain antibody for αvβ3 integrin in the microorganism.
Dependencies are inferred from the claim text.