Protoparvovirus compositions comprising a protoparvovirus variant VP1 capsid polypeptide and related methods
AssigneeCarbon Biosciences, Inc.
InventorsRobert Kotin, Sebastian Aguirre Kozlouski, Carolyn Pelletier
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Abstract
The present disclosure provides technologies comprising compositions, preparations, constructs, and methods comprising a protoparvovirus variant VP1 capsid polypeptide.
Claims21
A construct comprising a VP1 capsid coding sequence operably linked to an expression control sequence, wherein the VP1 capsid coding sequence encodes a protoparvovirus variant VP1 capsid polypeptide having an amino acid sequence that:
- (i) shows at least 98% sequence identity to SEQ ID NO:
- 104, or
- (ii) shows at least 98% sequence identity to SEQ ID NO:
- 107, wherein the protoparvovirus variant VP1 capsid polypeptide lacks an amino acid sequence as set forth in SEQ ID NO:
- 1.
A nucleic acid construct comprising a sequence that encodes a protoparvovirus variant VP1 capsid polypeptide, wherein the protoparvovirus variant VP1 capsid polypeptide has an amino acid sequence having at least 98% identity to SEQ ID NOs:
- 104 or 107, and wherein the protoparvovirus variant VP1 capsid polypeptide lacks an amino acid sequence as set forth in SEQ ID NO:
- 1.
A protoparvovirus variant VP1 capsid polypeptide having an amino acid sequence that:
- (i) shows at least 98% sequence identity to SEQ ID NO:
- 104, or
- (ii) shows at least 98% sequence identity to SEQ ID NO:
- 107,
- wherein the protoparvovirus variant VP1 capsid polypeptide lacks an amino acid sequence as set forth in SEQ ID NO:
- 1.
A virion comprising:
- (1) a protoparvovirus variant VP1 capsid polypeptide having an amino acid sequence that:
- (i) shows at least 98% sequence identity to SEQ ID NO:
- 104, or
- (ii) shows at least 98% sequence identity to SEQ ID NO:
- 107,
- wherein the protoparvovirus variant VP1 capsid polypeptide lacks an amino acid sequence as set forth in SEQ ID NO:
- 1;
- and
- (2) a heterologous nucleic acid sequence comprising:
- (i) a transgene coding sequence, and
- (ii) at least one inverted terminal repeat (ITR).
Dependencies are inferred from the claim text.