Biosensor and method for detection of analytes
AssigneeUniversity of Notre Dame du Lac
InventorsJiayun Hu, Paul W. Bohn, Marvin J. Miller, Manuka Ghosh
Life of this patent
Estimating the term…
Abstract
Described is a biosensor for detection of analytes and methods of using the same for detecting bacterial infection in a subject. The biosensor comprises an array of gold nanoparticles, biotinylated polyethylene glycol thiol, polyethylene glycol thiol, at least one neutravidin molecule, and at least one affinity reagent immobilized on a surface of the at least one neutravidin molecule. The affinity reagent may be an aptamer or a siderophore. The biosensors demonstrate extraordinary selectively and sensitivity for rapid detection of whole-cell bacteria.
Claims18
A biosensor for detection of analytes, the biosensor comprising:
- (a) an array of gold nanoparticles;
- (b) biotinylated polyethylene glycol thiol in direct contact with one or more of the gold nanoparticles;
- (c) polyethylene glycol thiol in direct contact with one or more of the gold nanoparticles;
- (d) at least one deglycosylated avidin molecule immobilized on a surface of the biotinylated polyethylene glycol thiol;
- and
- (e) at least one affinity reagent comprising a biotinylated aptamer encoded by a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:
- 1, the affinity reagent being immobilized on a surface of the at least one deglycosylated avidin molecule.
A method of detecting bacterial presence in a subject, the method comprising:
- (a) obtaining a sample from the subject;
- (b) contacting the sample with a biosensor, wherein the biosensor comprises:
- (i) an array of gold nanoparticles;
- (ii) biotinylated polyethylene glycol thiol in direct contact with one or more of the gold nanoparticles;
- (iii) polyethylene glycol thiol in direct contact with one or more of the gold nanoparticles;
- (iv) at least one deglycosylated avidin molecule immobilized on a surface of the biotinylated polyethylene glycol thiol;
- and
- (v) at least one affinity reagent comprising a biotinylated aptamer encoded by a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:
- 1, the affinity reagent being immobilized on a surface of the at least one deglycosylated avidin molecule;
- (c) detecting a localized surface plasmon resonance wavelength of the sample;
- and
- (d) comparing the localized surface plasmon resonance wavelength of the sample to a localized surface plasmon resonance wavelength in a control sample,
- wherein a shift in the wavelength of maximum extinction of the localized surface plasmon resonance of the sample relative to the wavelength of maximum extinction of the localized surface plasmon resonance of the control sample indicates a bacterial presence in the subject.
Dependencies are inferred from the claim text.