Methods for assessing cell surface glycosylation
AssigneeJuno Therapeutics, Inc.
InventorsPaul Ken Kodama, Tom Kowski, Mirna Mujacic, Kenneth Mayo Prentice
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Abstract
Provided herein are methods for assessing cell surface glycans, e.g., N-glycans, by assessing a sample of released surface glycans, and determining the presence, absence, or level of glycans present in the sample. Also provided are methods of assaying and/or evaluating a cell composition by assessing the cell surface glycan profile of the cell composition and comparing the profile to a reference sample. Methods for manufacturing and/or culturing a plurality of cell compositions having consistent surface glycan expression with low variability are also provided.
Claims18
A method of assaying a cell composition, the method comprising:
- (a) (i) assessing the cell surface profile in a sample from a test cell composition comprising a plurality of cells according to a method for assessing cell surface glycans comprising:
- (1) incubating the test cell composition comprising a plurality of cells under conditions to release one or more glycans from the surface of cells in the test cell composition, wherein a sample comprising one or more cell surface glycans is generated;
- and
- (2) determining the presence, absence, identity and/or level of glycans present in the sample, thereby assessing the cell surface glycan profile of the sample;
- and
- (a) (ii) comparing the cell surface glycan profile of the sample to the cell surface glycan profile of a reference sample;
- or
- (b) comparing the cell surface glycan profile of a sample with the cell surface profile of a reference sample, wherein cell surface glycan profile of the sample is or has been determined according to a method for assessing cell surface glycans comprising:
- (1) incubating a test cell composition comprising a plurality of cells under conditions to release one or more glycans from the surface of cells in the test cell composition, wherein a sample comprising one or more cell surface glycans is generated;
- and
- (2) determining the presence, absence, identity and/or level of glycans present in the sample, thereby assessing the cell surface glycan profile of the sample;
- and
- wherein the cells express a recombinant receptor or the test composition comprises cells expressing a recombinant receptor.
Dependencies are inferred from the claim text.