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US 12,379,357 B2Granted
Method for quantifying amino group-containing compound protected by protecting group having Fmoc skeleton
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsKiyoshi Sasakura, Keiji Nii
Life of this patent
Estimating the term…
Abstract
The present inventors discovered that compounds containing an amino group protected with a protecting group having an Fmoc skeleton can be quantitatively determined accurately by removing the protecting group having the Fmoc skeleton from the compounds and measuring the contents of dibenzofulvene or its derivative thereby produced.
Claims20
A method of quantitatively determining the content of a compound containing an amino group protected with a first protecting group having an Fmoc skeleton (hereinafter referred to as a first Fmoc compound) comprised in a crude product, the method comprising:
- deprotecting the first protecting group with a first base from the first Fmoc compound in a solution (hereinafter referred to as a first solution) to quantitatively determine the content of dibenzofulvene or its derivative thereby produced;
- and
- deprotecting a second protecting group having an Fmoc skeleton with a second base from a compound containing an amino group with the second protecting group (hereinafter referred to as a second solution) to quantitatively determine the content of dibenzofulvene or its derivative thereby produced.
Dependencies are inferred from the claim text.