Chromatography column and method of assembling the same

A method for fusing a component of a chromatography column assembly inside a chromatography column, the method comprising the steps of:

• a) providing a chromatography column cylinder having a wall including an inner surface, the wall being formed at least partially from thermoplastics material which is at least partially light energy transmissive and heat fusible, and partially defines a column volume;
• b) providing a first column component comprising an adapter plate having a sliding seal disposed therearound and a second column component providing a base inside the column cylinder;
• c) causing light energy to propagate through the light energy transmissive material of the wall toward the first column component, or the first column component and the second column component, the light energy being of sufficient magnitude to heat-fuse the heat fusible material of the first column component or the first column component and the second column component to the heat fusible material of the wall at a melt area where the propagation path of the light meets respective edge regions of the first column component or the first column component and the second column component;
• d) moving the propagation path relative to the wall and the first column component or the first column component and the second column component;
• and
• e) continuing step c) during said movement resulting from step d), or repeating step
• c) at further locations of the melt area.