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US 12,378,606 B2Granted
Probes and methods for measuring tandem repeats
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsWayne Frasch, Fusheng Xiong
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Abstract
The present disclosure relates to nucleic acid probes and kits for determining the length of a region of tandem repeats in a subject's genome and methods of using the DNA probes for determining the length of a region of tandem repeats in a subject's genome. In some embodiments, the region of tandem repeats in telomeres.
Claims20
A nucleic acid probe for the detection of a region of DNA, the probe comprising:
- a 5′ hybridization arm;
- a reverse PCR primer binding region;
- a forward PCR primer region;
- a minor groove binding (MGB) probe region;
- and
- a 3′ hybridization arm,
- wherein the sequences of the reverse PCR primer binding region and the MGB probe region each form a stem loop, and the 5′ hybridization arm and 3′ hybridization arm are complementary to adjacent regions in the region in DNA.
4 more elements
A method of determining the length of a region of tandem repeats in a DNA sample, the method comprising:
- hybridizing a nucleic acid probe to the DNA sample, the nucleic acid probe comprising:
- a 5′ hybridization arm;
- a reverse PCR primer binding region;
- a forward PCR primer region;
- a minor groove binding (MGB) probe region;
- and
- a 3′ hybridization arm,
- wherein the sequences of the reverse PCR primer binding region and the MGB probe region each form a stem loop and the 5′ hybridization arm and the 3′ hybridization arm of the nucleic acid probe are hybridized to adjacent regions in the region of tandem repeats in DNA;
- ligating the 5′ hybridization arm to the 3′ hybridization arm of the nucleic acid probe to form a circularized DNA;
- and
- quantifying the number of circularized DNA, wherein the number of circularized DNA correlates to the length of the region of tandem repeats in the DNA sample.
8 more elements
Dependencies are inferred from the claim text.