Multiplex Y-STR analysis
AssigneeLife Technologies Corporation
InventorsChristina Bormann Chung, Julio Mulero, Lori Hennessy
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Abstract
Novel Y-STR multiplex analysis designs, primer design, allelic ladders, methods of use and kits are disclosed, including the use of primer sets designed to provide amplicons for at least 11 Y-STR loci having a base pair size of less than about 220 bp, as well as the use of primer sets designed to provide amplicons for at least 22 Y-STR loci including at least 5 rapidly mutating loci.
Claims10
A kit for co-amplification of a set of loci in a DNA sample, the kit comprising a set of amplification primers provided together in the kit, the set of amplification primers comprising primers configured for the amplification of:
- Y-STR markers DYS576, DYS389I, DYS460, DYS458, DYS19, DYS456, DYS390, DYS570, DYS437, DYS393, and DYS439,
- wherein the kit omits one or more of Y-STR markers DYS385ab, DYS389II, DYS391, DYS392, DYS438, DYS448, or Y-GATA-H4, and
- wherein the kit comprises an allelic ladder that contains allelic size standards for known alleles of the Y-STR markers DYS576, DYS389I, DYS460, DYS458, DYS19, DYS456, DYS390, DYS570, DYS437, DYS393, and DYS439,
- wherein, for each Y-STR marker, the allelic size standards cover a range from a smallest allele to a largest allele amplified by the set of amplification primers configured to amplify the Y-STR marker,
- wherein each range of allelic size standards is separated by an at least two base pair difference from a preceding range of allelic size standards for a first adjacent Y-STR marker and a proceeding range of allelic size standards for a second adjacent Y-STR marker.
A kit for co-amplification of a set of loci in a DNA sample, the kit comprising a set of amplification primers provided together in the kit, the set of amplification primers comprising primers for the amplification of:
- (i) Y-STR markers DYS576, DYS389I, DYS460, DYS458, DYS19, DYS456, DYS390, DYS570, DYS437, DYS393, and DYS439;
- and
- (ii) at least 5-YSTR markers which are rapidly mutating, wherein the at least 5 rapidly mutating Y-STR markers comprise DYF387S1ab, DYS449, DYS570, DYS576, and DYS627, and optionally further comprise DYS518,
- wherein the kit omits one or more of Y-STR markers DYS385ab, DYS389II, DYS391, DYS392, DYS438, DYS448, or Y-GATA-H4, and
- wherein the kit comprises an allelic ladder that contains allelic size standards for known alleles of the Y-STR markers DYS576, DYS389I, DYS460, DYS458, DYS19, DYS456, DYS390, DYS570, DYS437, DYS393, and DYS439,
- wherein, for each Y-STR marker, the allelic size standards cover a range from a smallest allele to a largest allele amplified by the set of amplification primers configured to amplify the Y-STR marker,
- wherein each range of allelic size standards is separated by an at least two base pair difference from a preceding range of allelic size standards for a first adjacent Y-STR marker and a proceeding range of allelic size standards for a second adjacent Y-STR marker.
Dependencies are inferred from the claim text.