High-purity steviol glycosides
AssigneePureCircle USA Inc.
InventorsAvetik Markosyan, Siew Yin Chow, Khairul Nizam Bin Nawi, Kristina Chkhan, Mohamad Afzaal Bin Hasim, Saravanan A/L Ramandach
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Abstract
Methods of preparing highly purified steviol glycosides. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various starting compositions to target steviol glycosides. The highly purified steviol glycosides are useful as non-caloric sweetener, flavor enhancer, sweetness enhancer, and foaming suppressor in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.
Claims17
Steviol glycosides XV-XX and XXII-XXXIX with the following formulae:
- wherein R1 and R2 are sugar chains that are defined in the following table;
- No.
- R1
- XV
- XVI
- XVII
- XVIII
- XIX
- XX
- XXII
- XXIII
- XXIV
- XXV
- XXVI
- XXVII
- XXVIII
- XXIX
- XXX
- XXXI
- XXXII
- XXXIII
- XXXIV
- XXXV
- XXXVI
- XXXVII
- XXXVIII
- XXXIX
- No.
- R2
- XV
- XVI
- XVII
- XVIII
- XIX
- XX
- XXII
- XXIII
- XXIV
- XXV
- XXVI
- XXVII
- XXVIII
- XXIX
- XXX
- XXXI
- XXXII
- XXXIII
- XXXIV
- XXXV
- XXXVI
- XXXVII
- XXXVIII
- XXXIX
A method for producing at least one steviol glycoside with the formulae:
- wherein R1 and R2 are sugar chains that are defined in the following table;
- No.
- R1
- R2
- XV
- XVI
- XVII
- XVIII
- XIX
- XX
- XXII
- XXIII
- XXIV
- XXV
- XXVI
- XXVII
- XXVIII
- XXIX
- XXX
- XXXI
- XXXII
- XXXIII
- XXXIV
- XXXV
- XXXVI
- XXXVII
- XXXVIII
- XXXIX
- comprising the steps of:
- a) providing a starting composition comprising an organic compound with at least one carbon atom, wherein the starting composition is selected from the group consisting of steviol, steviolmonoside, steviolmonoside A, steviolbioside, steviolbioside D, rubusoside, steviolbioside A, steviolbioside B, rebaudioside B, stevioside, rebaudioside G, stevioside A, stevioside B, stevioside C, rebaudioside A, rebaudioside E, rebaudioside E2, rebaudioside E4, rebaudioside E6, rebaudioside E3, rebaudioside D, rebaudioside I, rebaudioside AM, rebaudioside D7, rebaudioside M, rebaudioside M4, rebaudioside M5, other steviol glycosides, polyols, carbohydrates, and combinations thereof;
- b) providing an enzyme preparation or a microorganism containing at least one enzyme selected from the group consisting of steviol biosynthesis enzymes, NDP-glucosyltransferases, and NDP-glucose recycling enzymes;
- c) contacting the enzyme preparation or microorganism with a medium containing the starting composition to produce a medium comprising the at least one steviol glycoside.
A method for producing at least one steviol glycoside with the formulae:
- wherein R1 and R2 are sugar chains that are defined in the following table;
- No.
- R1
- R2
- XV
- XVI
- XVII
- XVIII
- XIX
- XX
- XXII
- XXIII
- XXIV
- XXV
- XXVI
- XXVII
- XXVIII
- XXIX
- XXX
- XXXI
- XXXII
- XXXIII
- XXXIV
- XXXV
- XXXVI
- XXXVII
- XXXVIII
- XXXIX
- comprising the steps of:
- a) providing a starting composition comprising an organic compound with at least one carbon atom, wherein the starting composition is selected from the group consisting of steviol, steviolmonoside, steviolmonoside A, steviolbioside, steviolbioside D, rubusoside, steviolbioside A, steviolbioside B, rebaudioside B, stevioside, rebaudioside G, stevioside A, stevioside B, stevioside C, rebaudioside A, rebaudioside E, rebaudioside E2, rebaudioside E4, rebaudioside E6, rebaudioside E3, rebaudioside D, rebaudioside I, rebaudioside AM, rebaudioside D7, rebaudioside M, rebaudioside M4, rebaudioside M5, other steviol glycosides, polyols, carbohydrates, and combinations thereof;
- b) providing a biocatalyst comprising at least one enzyme selected from the group consisting of steviol biosynthesis enzymes, NDP-glucosyltransferases, and NDP-glucose recycling enzymes;
- c) contacting the biocatalyst with a medium containing the starting composition to produce a medium comprising the at least one steviol glycoside.
Dependencies are inferred from the claim text.