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US 12,378,580 B2Granted
Co-production pathway for 3-HPA and acetyl-CoA derivatives from malonate semialdehyde
AssigneeBraskem S.A.
InventorsBeatriz Leite Magalhaes, Paulo Moises Raduan Alexandrino, Felipe Galzerani
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Abstract
The present disclosure provides methods for utilizing genetically modified microbes to co-produce 3-hydroxypropionic acid (3-HP) and acetyl-CoA, and derivatives thereof from malonate semialdehyde as a common single intermediate. The disclosure further provides modified microbe that co-produce the 3-HP and acetyl-CoA derivatives from malonate semialdehyde.
Claims22
A recombinant microorganism capable of producing acetone and/or isopropanol from a feedstock comprising one or more carbon sources, wherein the recombinant microorganism comprises:
- (a) at least one endogenous and/or exogenous nucleic acid molecule encoding an aspartate amino transferase that catalyzes the production of aspartate from oxaloacetate;
- (b) at least one exogenous nucleic acid molecule encoding an aspartate decarboxylase that catalyzes the production of β-alanine from aspartate from (a);
- (c) at least one endogenous and/or exogenous nucleic acid molecule encoding a β-alanine pyruvate amino transferase and/or a β-alanine transaminase that catalyzes the conversion of β-alanine from (b) to malonate semialdehyde;
- and
- (d) a pathway for the conversion of malonate semialdehyde to acetone.
2 more elements
Dependencies are inferred from the claim text.