Chemically-defined baculovirus expression system
AssigneeLife Technologies Corporation
InventorsMaya Yovcheva, Jonathan Zmuda, Sara Barnes, Chang Choi, Kenneth Thompson
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Abstract
The present disclosure is related generally to systems and methods for high level expression of recombinant proteins from baculovirus in insect cells. In particular, the methods and systems described herein allow for high levels of baculovirus production in insect cells and/or high levels of protein production in insect cells using a chemically-defined, yeast lysate-free insect cell medium. The disclosure also relates to compositions and kits for culturing, transfecting, and/or producing recombinant protein in insect cells.
Claims18
A baculovirus expression system comprising:
- (a) an insect cell medium, wherein the insect cell medium comprises an energy source selected from maltose, sucrose, glucose, trehalose, fructose, mannose, lactose, galactose, dextrose, or combinations thereof;
- and
- (b) a plurality of Sf9 cells.
Dependencies are inferred from the claim text.