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US 12,378,574 B2Granted
Transgenic mouse models supporting innate immune function
AssigneeThe Jackson Laboratory
InventorLeonard D. Shultz
Life of this patent
Estimating the term…
Abstract
Provided herein, in some aspects, is a NOD.Cg-Prkdcscid Il2rgtm1wjl/SzJ (NOD scid gamma or NSG™) mouse comprising a nucleic acid encoding human FLT3L and an inactivated mouse Flt3 allele, methods of producing the mouse, and methods of using the mouse.
Claims20
A NOD.Cg-Prkdcscid Il2rgtm1wjl/SzJ (NOD scid gamma) mouse whose genome comprises
- a human FLT3L transgene and
- an inactivated mouse Flt3 allele.
A method comprising:
- injecting a pronucleus of a NOD.Cg-Prkdcscid Il2rgtm1wjl/SzJ (NOD scid gamma (NSG)) mouse with a nucleic acid encoding human FLT3L, producing a NSG Tg(Hu-FLT3L) mouse, and inactivating a mouse Flt3 allele in the NSG Tg(Hu-FLT3L) mouse;
- or
- inactivating a mouse Flt3 allele in a NOD.Cg-Prkdcscid Il2rgtm1wjl/SzJ (NOD scid gamma) mouse to produce a NSG Flt3null mouse, and injecting a pronucleus of the NSG Flt3null mouse with a nucleic acid encoding human FLT3L to produce a NSG Flt3null Tg(Hu-FLT3L) mouse.
A NOD.Cg-Prkdcscid Il2rgtm1wjl/SzJ cell comprising a nucleic acid encoding human FLT3L and an inactivated endogenous Flt3 allele.
Dependencies are inferred from the claim text.