Production of carotenoids and apocarotenoids
AssigneeAgency for Science, Technology and Research
InventorsCongqiang Zhang, Xixian Chen, Heng-Phon Too
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Abstract
A method for producing a carotenoid or apocarotenoid is disclosed. The method comprises the step of expressing in a host cell an expression module comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter. A host cell comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter is also provided together with a kit.
Claims10
A method for producing an apocarotenoid, wherein the apocarotenoid is retinal, retinol, or a combination of retinal and retinol, comprising the step of expressing in an isolated Escherichia coli host cell an expression module comprising an expression vector having a coding region encoding blh (β-carotene dioxygenase) from uncultured marine bacterium HF10 19P19, wherein the coding region is codon optimized and operably linked to a promoter, and wherein the isolated host cell further expresses ybbO (retinol dehydrogenase) from Escherichia coli.
Dependencies are inferred from the claim text.