US 12,378,559 B2Granted

Enzymatic nucleic acid molecules

AssigneeUniversity of Essex Enterprises Limited

InventorsGreg Brooke, Antonio Marco Castillo, Angela Pine

Life of this patent

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Abstract

Certain aspects of the present invention relate to methods and reagents for the treatment of prostate cancer. Also included in the present invention is an enzymatic nucleic acid molecule (“DNAzyme”) comprising a catalytic core and two specific binding arms complementary to a nucleic acid sequence comprised in an Androgen Receptor mRNA molecule. Also included are compositions comprising the enzymatic nucleic acid molecules and methods of treating disorders such as prostate cancer comprising use of such nucleic acid molecules.

Claims10

Dependent claims

An enzymatic nucleic acid molecule which regulates the expression of a human Androgen Receptor gene, wherein the enzymatic nucleic acid molecule comprises a nucleic acid sequence having at least 80% sequence identity to a nucleic acid sequence selected from:

a. The nucleic acid sequence set forth in SEQ. ID. No 1 (DZAR3)
b. The nucleic acid sequence set forth in SEQ. ID. No 2 (DZAR18)
c. The nucleic acid sequence set forth in SEQ. ID. No 3 (DZAR15)
d. The nucleic acid sequence set forth in SEQ. ID. No 4 (DZAR8)
e. The nucleic acid sequence set forth in SEQ. ID. No 5 (DZAR34)
f. The nucleic acid sequence set forth in SEQ. ID. No 6 (DZAR24)
g. The nucleic acid sequence set forth in SEQ. ID. No 7 (DZAR23)
h. The nucleic acid sequence set forth in SEQ. ID. No 8 (DZAR28)
i. The nucleic acid sequence set forth in SEQ. ID. No 9 (DZAR27)
j. The nucleic acid sequence set forth in SEQ. ID. No 10 (DZAR25)
k. The nucleic acid sequence set forth in SEQ. ID. No 11 (DZAR7)
l. The nucleic acid sequence set forth in SEQ. ID. No 12 (DZAR29)
m. The nucleic acid sequence set forth in SEQ. ID. No 13 (DZAR5)
n. The nucleic acid sequence set forth in SEQ. ID. No 14 (DZAR9)
o. The nucleic acid sequence set forth in SEQ. ID. No 15 (DZAR19)
p. The nucleic acid sequence set forth in SEQ. ID. No 16 (DZAR14)
q. The nucleic acid sequence set forth in SEQ. ID. No 17 (DZAR21)
r. The nucleic acid sequence set forth in SEQ. ID. No 18 (DZAR33)
s. The nucleic acid sequence set forth in SEQ. ID. No 19 (DZAR13)
t. The nucleic acid sequence set forth in SEQ. ID. No 20 (DZAR16)
u. The nucleic acid sequence set forth in SEQ. ID. No 21 (DZAR1)
v. The nucleic acid sequence set forth in SEQ. ID. No 22 (DZAR20)
w. The nucleic acid sequence set forth in SEQ. ID. No 23 (DZAR2)
x. The nucleic acid sequence set forth in SEQ. ID. No 24 (DZAR26)
y. The nucleic acid sequence set forth in SEQ. ID. No 25 (DZAR4)
Z. The nucleic acid sequence set forth in SEQ. ID. No 26 (DZAR10)
aa. The nucleic acid sequence set forth in SEQ. ID. No 27 (DZAR11)
bb. The nucleic acid sequence set forth in SEQ. ID. No 28 (DZAR12)
cc. The nucleic acid sequence set forth in SEQ. ID. No 29 (DZAR17)
dd. The nucleic acid sequence set forth in SEQ. ID. No 30 (DZAR22)
ee. The nucleic acid sequence set forth in SEQ. ID. No 31 (DZAR30)
ff. The nucleic acid sequence set forth in SEQ. ID. No 32 (DZAR31)
gg. The nucleic acid sequence set forth in SEQ. ID. No 33 (DZAR32);
and/or the enzymatic nucleic acid molecule consists of a nucleic acid sequence having at least 80% sequence identity to a nucleic acid sequence selected from:
a. The nucleic acid sequence set forth in SEQ. ID. No 1 (DZAR3)
b. The nucleic acid sequence set forth in SEQ. ID. No 2 (DZAR18)
c. The nucleic acid sequence set forth in SEQ. ID. No 3 (DZAR15)
d. The nucleic acid sequence set forth in SEQ. ID. No 4 (DZAR8)
e. The nucleic acid sequence set forth in SEQ. ID. No 5 (DZAR34)
f. The nucleic acid sequence set forth in SEQ. ID. No 6 (DZAR24)
g. The nucleic acid sequence set forth in SEQ. ID. No 7 (DZAR23)
h. The nucleic acid sequence set forth in SEQ. ID. No 8 (DZAR28)
i. The nucleic acid sequence set forth in SEQ. ID. No 9 (DZAR27)
j. The nucleic acid sequence set forth in SEQ. ID. No 10 (DZAR25)
k. The nucleic acid sequence set forth in SEQ. ID. No 11 (DZAR7)
l. The nucleic acid sequence set forth in SEQ. ID. No 12 (DZAR29)
m. The nucleic acid sequence set forth in SEQ. ID. No 13 (DZAR5)
n. The nucleic acid sequence set forth in SEQ. ID. No 14 (DZAR9)
o. The nucleic acid sequence set forth in SEQ. ID. No 15 (DZAR19)
p. The nucleic acid sequence set forth in SEQ. ID. No 16 (DZAR14)
q. The nucleic acid sequence set forth in SEQ. ID. No 17 (DZAR21)
r. The nucleic acid sequence set forth in SEQ. ID. No 18 (DZAR33)
s. The nucleic acid sequence set forth in SEQ. ID. No 19 (DZAR13)
t. The nucleic acid sequence set forth in SEQ. ID. No 20 (DZAR16)
u. The nucleic acid sequence set forth in SEQ. ID. No 21 (DZAR1)
v. The nucleic acid sequence set forth in SEQ. ID. No 22 (DZAR20)
w. The nucleic acid sequence set forth in SEQ. ID. No 23 (DZAR2)
x. The nucleic acid sequence set forth in SEQ. ID. No 24 (DZAR26)
y. The nucleic acid sequence set forth in SEQ. ID. No 25 (DZAR4)
z. The nucleic acid sequence set forth in SEQ. ID. No 26 (DZAR10)
aa. The nucleic acid sequence set forth in SEQ. ID. No 27 (DZAR11)
bb. The nucleic acid sequence set forth in SEQ. ID. No 28 (DZAR12)
cc. The nucleic acid sequence set forth in SEQ. ID. No 29 (DZAR17)
dd. The nucleic acid sequence set forth in SEQ. ID. No 30 (DZAR22)
ee. The nucleic acid sequence set forth in SEQ. ID. No 31 (DZAR30)
ff. The nucleic acid sequence set forth in SEQ. ID. No 32 (DZAR31)
gg. The nucleic acid sequence set forth in SEQ. ID. No 33 (DZAR32).

64 more elements

Dependencies are inferred from the claim text.

Facts

Application: 17/290,119Filed 31 Oct 2019
Priority: GB 18179902 Nov 2018
Published: US 2021/0395753 A123 Dec 2021
Granted: 5 Aug 2025
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

C07K 14/721
For hormones: steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
C12N 15/1138
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming…: against…
A61P 35/00
Antineoplastic agents
C12N 2310/127
Catalytic nucleic acids, e.g. ribozymes: dNAzymes

Family

US 2021/0395753 A1Pre-grant publication · published 23 Dec 2021
US 12,378,559 B2This patent

From the US filing history. Foreign family members aren’t listed.

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Abstract

a. The nucleic acid sequence set forth in SEQ. ID. No 1 (DZAR3)
b. The nucleic acid sequence set forth in SEQ. ID. No 2 (DZAR18)
c. The nucleic acid sequence set forth in SEQ. ID. No 3 (DZAR15)
d. The nucleic acid sequence set forth in SEQ. ID. No 4 (DZAR8)
e. The nucleic acid sequence set forth in SEQ. ID. No 5 (DZAR34)
f. The nucleic acid sequence set forth in SEQ. ID. No 6 (DZAR24)
g. The nucleic acid sequence set forth in SEQ. ID. No 7 (DZAR23)
h. The nucleic acid sequence set forth in SEQ. ID. No 8 (DZAR28)
i. The nucleic acid sequence set forth in SEQ. ID. No 9 (DZAR27)
j. The nucleic acid sequence set forth in SEQ. ID. No 10 (DZAR25)
k. The nucleic acid sequence set forth in SEQ. ID. No 11 (DZAR7)
l. The nucleic acid sequence set forth in SEQ. ID. No 12 (DZAR29)
m. The nucleic acid sequence set forth in SEQ. ID. No 13 (DZAR5)
n. The nucleic acid sequence set forth in SEQ. ID. No 14 (DZAR9)
o. The nucleic acid sequence set forth in SEQ. ID. No 15 (DZAR19)
p. The nucleic acid sequence set forth in SEQ. ID. No 16 (DZAR14)
q. The nucleic acid sequence set forth in SEQ. ID. No 17 (DZAR21)
r. The nucleic acid sequence set forth in SEQ. ID. No 18 (DZAR33)
s. The nucleic acid sequence set forth in SEQ. ID. No 19 (DZAR13)
t. The nucleic acid sequence set forth in SEQ. ID. No 20 (DZAR16)
u. The nucleic acid sequence set forth in SEQ. ID. No 21 (DZAR1)
v. The nucleic acid sequence set forth in SEQ. ID. No 22 (DZAR20)
w. The nucleic acid sequence set forth in SEQ. ID. No 23 (DZAR2)
x. The nucleic acid sequence set forth in SEQ. ID. No 24 (DZAR26)
y. The nucleic acid sequence set forth in SEQ. ID. No 25 (DZAR4)
Z. The nucleic acid sequence set forth in SEQ. ID. No 26 (DZAR10)
aa. The nucleic acid sequence set forth in SEQ. ID. No 27 (DZAR11)
bb. The nucleic acid sequence set forth in SEQ. ID. No 28 (DZAR12)
cc. The nucleic acid sequence set forth in SEQ. ID. No 29 (DZAR17)
dd. The nucleic acid sequence set forth in SEQ. ID. No 30 (DZAR22)
ee. The nucleic acid sequence set forth in SEQ. ID. No 31 (DZAR30)
ff. The nucleic acid sequence set forth in SEQ. ID. No 32 (DZAR31)
gg. The nucleic acid sequence set forth in SEQ. ID. No 33 (DZAR32);
and/or the enzymatic nucleic acid molecule consists of a nucleic acid sequence having at least 80% sequence identity to a nucleic acid sequence selected from:
a. The nucleic acid sequence set forth in SEQ. ID. No 1 (DZAR3)
b. The nucleic acid sequence set forth in SEQ. ID. No 2 (DZAR18)
c. The nucleic acid sequence set forth in SEQ. ID. No 3 (DZAR15)
d. The nucleic acid sequence set forth in SEQ. ID. No 4 (DZAR8)
e. The nucleic acid sequence set forth in SEQ. ID. No 5 (DZAR34)
f. The nucleic acid sequence set forth in SEQ. ID. No 6 (DZAR24)
g. The nucleic acid sequence set forth in SEQ. ID. No 7 (DZAR23)
h. The nucleic acid sequence set forth in SEQ. ID. No 8 (DZAR28)
i. The nucleic acid sequence set forth in SEQ. ID. No 9 (DZAR27)
j. The nucleic acid sequence set forth in SEQ. ID. No 10 (DZAR25)
k. The nucleic acid sequence set forth in SEQ. ID. No 11 (DZAR7)
l. The nucleic acid sequence set forth in SEQ. ID. No 12 (DZAR29)
m. The nucleic acid sequence set forth in SEQ. ID. No 13 (DZAR5)
n. The nucleic acid sequence set forth in SEQ. ID. No 14 (DZAR9)
o. The nucleic acid sequence set forth in SEQ. ID. No 15 (DZAR19)
p. The nucleic acid sequence set forth in SEQ. ID. No 16 (DZAR14)
q. The nucleic acid sequence set forth in SEQ. ID. No 17 (DZAR21)
r. The nucleic acid sequence set forth in SEQ. ID. No 18 (DZAR33)
s. The nucleic acid sequence set forth in SEQ. ID. No 19 (DZAR13)
t. The nucleic acid sequence set forth in SEQ. ID. No 20 (DZAR16)
u. The nucleic acid sequence set forth in SEQ. ID. No 21 (DZAR1)
v. The nucleic acid sequence set forth in SEQ. ID. No 22 (DZAR20)
w. The nucleic acid sequence set forth in SEQ. ID. No 23 (DZAR2)
x. The nucleic acid sequence set forth in SEQ. ID. No 24 (DZAR26)
y. The nucleic acid sequence set forth in SEQ. ID. No 25 (DZAR4)
z. The nucleic acid sequence set forth in SEQ. ID. No 26 (DZAR10)
aa. The nucleic acid sequence set forth in SEQ. ID. No 27 (DZAR11)
bb. The nucleic acid sequence set forth in SEQ. ID. No 28 (DZAR12)
cc. The nucleic acid sequence set forth in SEQ. ID. No 29 (DZAR17)
dd. The nucleic acid sequence set forth in SEQ. ID. No 30 (DZAR22)
ee. The nucleic acid sequence set forth in SEQ. ID. No 31 (DZAR30)
ff. The nucleic acid sequence set forth in SEQ. ID. No 32 (DZAR31)
gg. The nucleic acid sequence set forth in SEQ. ID. No 33 (DZAR32).

64 more elements

Facts

Application

17/290,119Filed 31 Oct 2019

Priority

GB 18179902 Nov 2018

Published

US 2021/0395753 A123 Dec 2021

Granted

5 Aug 2025

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C07K 14/721
For hormones: steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
C12N 15/1138
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming…: against…
A61P 35/00
Antineoplastic agents
C12N 2310/127
Catalytic nucleic acids, e.g. ribozymes: dNAzymes