Method for nucleic acid depletion
AssigneeUEA Enterprises Limited
InventorsJustin Joseph O'Grady, John Richard Wain, Solomon Mwaigwisya, Gemma Louise Kay
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Abstract
Provided is a method for depleting host nucleic acid in a biological sample, said sample having been previously obtained from an animal host, said method comprising the steps of (a) adding a cytolysin, or an active variant thereof, to said sample; and (b) carrying-out a process to physically deplete nucleic acid released from host cells within said sample or otherwise render such nucleic acid unidentifiable.
Claims16
A method for depleting host nucleic acid in a biological sample while maintaining non-host nucleic acid, said sample having been previously obtained from an animal host, said method comprising the steps of:
- (a) adding a phospholipase D (PLD), or an active variant thereof, to said sample to selectively lyse host cells in said biological sample;
- and
- (b) carrying-out a process to physically deplete the host nucleic acid released from the lysed host cells within said biological sample,
- wherein the active variant is a variant of a wild-type PLD that has at least 10% of the lytic activity of the wild-type PLD.
Dependencies are inferred from the claim text.