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US 12,378,541 B2Granted
Penicillin-G acylases
AssigneeCodexis, Inc.
InventorsGoutami Banerjee, Jie Yang, Xiyun Zhang, Erika M. Milczek, Melissa Ann Mayo, Stephan Jenne
Life of this patent
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Abstract
The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
Claims3
A method for producing free insulin, comprising:
- i) providing an engineered penicillin G acylase capable of removing A1/B1/B29 tri-phenyl acetate protecting groups from insulin to produce free insulin, wherein said penicillin G acylase is at least 95% identical to SEQ ID NO:
- 6, and wherein said engineered penicillin G acylase comprises an alanine at position 264, an asparagine at position 484, and a lysine at position 547, wherein said positions are numbered with reference to SEQ ID NO:
- 6 and insulin comprising A1/B1/B29 tri-phenyl acetate protecting groups;
- and ii) exposing said engineered penicillin G acylase to said insulin comprising A1/B1/B29 tri-phenyl acetate protecting groups under conditions such that said engineered penicillin G acylase removes the A1/B1/B29 tri-phenyl acetate protecting groups and free insulin is produced.
1 more element
Dependencies are inferred from the claim text.