Phytase mutant
AssigneeQingdao Vland Biotech Group Co., Ltd.
InventorsXiuxiu Wu, Yijun Huang, Yang Liu, Xia Zhang, Sida Cheng
Life of this patent
Estimating the term…
Abstract
The present invention relates to the technical field of biology, in particular to a phytase mutant, a preparation method therefor and an application thereof, a DNA molecule encoding the phytase mutant, a vector, and a host cell. The mutant provided by the present invention contains the substituent of an amino acid at at least one position selected from the following group: 36, 69, 89, 91, 111, 202, 213, 225, 238, 243, 253, 258, and 266. The heat resistance of the mutant is significantly improved, thereby facilitating the wide application of the phytase in feed.
Claims8
A phytase mutant, wherein the mutant comprises an amino acid sequence having at least 90% identity with SEQ ID NO:
- 1, and the mutant comprises an amino acid substitution or a combination selected from the group consisting of:
- Q258E,
- Q258E/S266P,
- V89T/Q258E,
- E91Q/Q258E,
- Q225Y/Q258E,
- Q253Y/Q258E,
- V89T/E91Q/Q258E,
- V89T/Q225Y/Q258E,
- V89T/Q253Y/Q258E,
- V89T/Q258E/S266P,
- E91Q/Q225Y/Q258E,
- E91Q/Q253Y/Q258E,
- E91Q/Q258E/S266P,
- Q225Y/Q253Y/Q258E,
- Q225Y/Q258E/S266P,
- V89T/E91Q/Q225Y/Q258E,
- V89T/E91Q/Q253Y/Q258E,
- V89T/E91Q/Q258E/S266P,
- E91Q/Q225Y/Q253Y/Q258E,
- E91Q/Q225Y/Q258E/S266P,
- V89T/Q225Y/Q253Y/Q258E,
- V89T/Q225Y/Q258E/S266P,
- E91Q/Q225Y/Q253Y/Q258E,
- E91Q/Q225Y/Q258E/S266P,
- E91Q/Q253Y/Q258E/S266P,
- V89T/Q253Y/Q258E/S266P,
- V89T/E91Q/Q225Y/Q253Y/Q258E,
- V89T/Q225Y/Q253Y/Q258E/S266P,
- V89T/E91Q/Q253Y/Q258E/S266P,
- V89T/E91Q/Q225Y/Q258E/S266P,
- E91Q/Q225Y/Q253Y/Q258E/S266P,
- V89T/E91Q/Q225Y/Q253Y/Q258E/S266P,
- S266P,
- V89T/S266P,
- E91Q/S266P,
- Q225Y/S266P,
- Q253Y/S266P,
- V89T/E91Q/S266P,
- V89T/Q225Y/S266P,
- V89T/Q253Y/S266P,
- E91Q/Q225Y/S266P,
- E91Q/Q253Y/S266P,
- Q225Y/Q253Y/S266P,
- V89T/E91Q/Q225Y/S266P,
- V89T/E91Q/Q253Y/S266P,
- V89T/Q225Y/Q253Y/S266P,
- V89T/E91Q/Q225Y/Q253Y/S266P,
- V89T/E91Q,
- V89T/Q253Y,
- E91Q/Q253Y,
- V89T/E91Q/Q253Y,
- V89T/E91Q/Q225Y,
- V89T/Q225Y/Q253Y,
- E91Q/Q225Y/Q253Y,
- V89T/E91Q/Q225Y/Q253Y,
- V89T/E91Q/A202P/Q253Y,
- V89T/E91Q/L213F/Q253Y,
- V89T/E91Q/L213W/Q253Y,
- V89T/E91Q/W243V/Q253Y,
- V89T/E91Q/W243L/Q253Y,
- D69F/V89T/E91Q/Q253Y,
- D69Q/V89T/E91Q/Q253Y,
- V89T/E91Q/T111P/Q253Y,
- V89T/E91Q/T238R/Q253Y,
- A36P/V89T/E91Q/Q253Y,
- V89T/E91Q/A202P/L213F/Q253Y,
- V89T/E91Q/A202P/L213W/Q253Y,
- V89T/E91Q/A202P/W243V/Q253Y,
- V89T/E91Q/A202P/W243L/Q253Y,
- V89T/E91Q/L213F/W243V/Q253Y,
- V89T/E91Q/L213W/W243L/Q253Y,
- V89T/E91Q/L213F/W243L/Q253Y,
- V89T/E91Q/L213W/W243V/Q253Y,
- V89T/E91Q/A202P/L213F/W243V/Q253Y,
- V89T/E91Q/A202P/L213W/W243L/Q253Y,
- V89T/E91Q/A202P/L213F/W243L/Q253Y,
- V89T/E91Q/A202P/L213W/W243V/Q253Y,
- V89T/E91Q/A202P/Q225Y/Q253Y,
- V89T/E91Q/L213F/Q225Y/Q253Y,
- V89T/E91Q/L213W/Q225Y/Q253Y,
- V89T/E91Q/W243V/Q225Y/Q253Y,
- V89T/E91Q/W243L/Q225Y/Q253Y,
- D69F/V89T/E91Q/Q225Y/Q253Y,
- D69Q/V89T/E91Q/Q225Y/Q253Y,
- V89T/E91Q/T111P/Q225Y/Q253Y,
- V89T/E91Q/T238R/Q225Y/Q253Y,
- A36P/V89T/E91Q/Q225Y/Q253Y,
- V89T/E91Q/A202P/Q225Y/Q253Y/Q258E/S266P,
- V89T/E91Q/L213F/Q225Y/Q253Y/Q258E/S266P,
- V89T/E91Q/L213W/Q225Y/Q253Y/Q258E/S266P,
- V89T/E91Q/W243V/Q225Y/Q253Y/Q258E/S266P,
- V89T/E91Q/W243L/Q225Y/Q253Y/Q258E/S266P,
- D69F/V89T/E91Q/Q225Y/Q253Y/Q258E/S266P,
- D69Q/V89T/E91Q/Q225Y/Q253Y/Q258E/S266P,
- V89T/E91Q/T111P/Q225Y/Q253Y/Q258E/S266P,
- V89T/E91Q/T238R/Q225Y/Q253Y/Q258E/S266P,
- A36P/V89T/E91Q/Q225Y/Q253Y/Q258E/S266P,
- V89T/E91Q/A202P/L213F/Q225Y/Q253Y/Q258E/S266P,
- V89T/E91Q/A202P/L213W/Q225Y/Q253Y/Q258E/S266P,
- V89T/E91Q/A202P/Q225Y/W243V/Q253Y/Q258E/S266P,
- V89T/E91Q/A202P/Q225Y/W243L/Q253Y/Q258E/S266P,
- V89T/E91Q/L213F/Q225Y/W243V/Q253Y/Q258E/S266P,
- V89T/E91Q/L213W/Q225Y/W243L/Q253Y/Q258E/S266P,
- V89T/E91Q/L213F/Q225Y/W243L/Q253Y/Q258E/S266P,
- V89T/E91Q/L213W/Q225Y/W243V/Q253Y/Q258E/S266P,
- V89T/E91Q/A202P/L213F/Q225Y/W243V/Q253Y/Q258E/S266P,
- V89T/E91Q/A202P/L213W/Q225Y/W243L/Q253Y/Q258E/S266P,
- V89T/E91Q/A202P/L213F/Q225Y/W243L/Q253Y/Q258E/S266P,
- V89T/E91Q/A202P/L213W/Q225Y/W243V/Q253Y/Q258E/S266P,
- A202P,
- L213F,
- L213W,
- W243V,
- W243L,
- D69Q,
- D69F,
- T111P,
- T238R,
- A36P,
- A202P/L213F,
- A202P/L213W,
- A202P/W243L,
- A202P/W243V,
- A202P/L213F/W243L,
- A202P/L213F/W243V,
- A202P/L213W/W243V, and
- A202P/L213W/W243L.
Dependencies are inferred from the claim text.