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US 12,378,509 B2Granted
Method for producing cell cluster group and device for producing same
AssigneeJsr Corporation
InventorYuki Yokokawa
Life of this patent
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Abstract
This method for producing a cell cluster group comprises: a step for putting, into a well, a cell suspension obtained by suspending dispersed cells in a medium, using a cell incubator which includes the well and two or more recesses formed in the bottom of the well and in which the area of an opening of each recess in plan view is at most 1 mm2; a step for centrifuging the cell incubator; and a step for culturing the dispersed cells in the recesses.
Claims10
A method for production of a cell cluster group, the method comprising:
- adding into a well of a cell-cultivation container a cell suspension in which dispersed cells are suspended in a culture medium, wherein the well further comprises at least two recesses formed in a bottom face of the well, and wherein an area of an opening of each of the at least two recesses in plan view is 1 mm2 or less;
- centrifuging the cell-cultivation container;
- and
- culturing the dispersed cells in the recesses,
- wherein an inner wall surface of each of the at least two recesses is coated with a cell-non-adhesiveness-imparting agent;
- and
- wherein each of the at least two recesses has a cylindrical shape or a conical shape.
4 more elements
Dependencies are inferred from the claim text.