Ehrlichia ruminantium immunogenic compositions and methods of using thereof
AssigneeKansas State University Research Foundation
InventorsBonto Faburay, Jodi McGill
Life of this patent
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Abstract
The present disclosure provides compositions and methods for reducing the incidence of and/or severity of diseases associated with tick-borne pathogens. In preferred forms, the compositions comprise a recombinant antigenic protein subunit that has been glycosylated. Some preferred subunits include the MAP1 protein of Ehrlichia ruminantium, the p30-1 sequence from Ehrlichia canis, the p28-Omp19 protein from Ehrlichia chaffeensis, and the MSP4 protein from Anaplasma marginale. Administration of such compositions to an animal in need thereof provides protection against clinical signs of infection in susceptible animals.
Claims12
A composition comprising a recombinant antigenic protein subunit from a tick-borne pathogen selected from the group consisting of Ehrlichia ruminantium, Ehrlichia chaffeensis, Ehrlichia canis, Ehrlichia ewingii, Ehrlichia muris, Anaplasma phagocytophilum, Anaplasma platys, Anaplasma marginale, and any combination thereof, wherein the subunit has been glycosylated, and further comprising the MAP1 signal peptide from Ehrlichia ruminantium, the adjuvant montanide ISA25, and an additional element selected from the group consisting of at least one additional antigen from a pathogen other than one of said tick-borne pathogens, a preservative, a stabilizer, a color, a flavor, and any combination thereof, wherein the recombinant antigenic protein subunit has been glycosylated from expression through a mammalian or eukaryotic system, and wherein said recombinant antigenic protein subunit has at least 90% sequence homology with a sequence selected from the group consisting of SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 12, SEQ ID No. 13, and any combination thereof.
A composition comprising a recombinant antigenic protein subunit from a tick-borne pathogen selected from the group consisting of Ehrlichia ruminantium, Ehrlichia chaffeensis, Ehrlichia canis, Ehrlichia ewingii, Ehrlichia muris, Anaplasma phagocytophilum, Anaplasma platys, Anaplasma marginale, and any combination thereof, wherein the subunit has been glycosylated, the adjuvant Montanide ISA25, and further comprising the MAP1 signal peptide from Ehrlichia ruminantium and an additional element selected from the group consisting of at least one additional antigen from a pathogen other than one of said tick-borne pathogens, a preservative, a stabilizer, a color, a flavor, and any combination thereof, wherein the recombinant antigenic protein subunit has been glycosylated from expression through Spodoptera frugiperda (Sf9) insect cells, and wherein said recombinant antigenic protein subunit has at least 90% sequence homology with a sequence selected from the group consisting of SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 12, SEQ ID No. 13, and any combination thereof.
Dependencies are inferred from the claim text.