In-vitro culture, induction, activation and cryopreservation method and cell bank establishment for immune cells

An in-vitro culture, induction, activation and cryopreservation method and cell bank establishment for immune cells, comprising the followings:

• using a dedicated amplification medium of immune cells to perform first-stage amplification culture on mononuclear cells to obtain preliminarily amplified immune cells;
• wherein the dedicated amplification medium of immune cells is a serum-free lymphocyte medium added with 500-2000 IU/ml IL-2, 500-2000 IU/ml IL-10, 0.5-1 ng/ml IL-1α and 1-4 ng/ml LIF, 0.5-2.5 ng/ml EPO, 1-4 ng/ml KGF, 2-5 ng/ml testosterone, 1-4 μg/ml parathyroid hormone and 1-4 μg/ml laminin;
• using a dedicated induction medium of immune cells to perform second-stage induction and amplification culture on the preliminarily amplified immune cells to obtain induced immune cells;
• using a dedicated activation medium of immune cells to perform third-stage activation and amplification culture on the induced immune cells to obtain a large number of immune cells with activation functions;
• using a dedicated cryopreserving fluid of immune cells to cryopreserve the immune cells to obtain cryopreserved immune cells;
• and performing preservation according to ABO/RH typing and HLA typing;
• and establishing an information file of immune cells for retrieval to construct an immune cell bank;
• wherein the immune cells are natural killer cells.