Systems and methods for quantifying an analyte extracted from a sample

A method for analyzing an enzymatic reaction, the method comprising:

• introducing a solvent into a capillary;
• introducing the capillary into a vessel comprising a sample comprising an enzyme and a reactant such that a portion of the sample comprising the enzyme and the reactant is introduced into the capillary;
• allowing the enzyme and the reactant to react with each other in the capillary to form a reaction product, which reaction continues over time along with formation of the reaction product over time;
• moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent while the sample and the solvent remain separate from each other, thereby causing the reaction product to be extracted from the sample and into the solvent;
• analyzing a first amount of formation of the reaction product that has been extracted from the sample at a first time point;
• and
• repeating the moving and analyzing steps at least one more time to analyze at least a second amount of formation of the reaction product at least at a second time point, thereby monitoring reaction product formation over time.