Back to results
US 12,372,537 B2Granted
Determination of Parkinson's disease
AssigneeHoffmann-La Roche Inc.
InventorsChristian Czech, Sebastian Dziadek, Lidija Pestic-Dragovich, Lei Tang, Thomas Kremer, Wagner Marcelo Zago, Tsu-Shuen Tsao, Adriana Racolta, Marta Canamero, Ronald Torres, Mirko Ritter
Life of this patent
Estimating the term…
Abstract
Methods and compositions for accurate identification of Parkinson's disease are disclosed. More particularly, the disclosure is directed to the determination of Parkinson's disease in ante-mortem tissue samples.
Claims30
A method for determining whether a subject has Parkinson's Disease (PD), the method comprising:
- (a) contacting a biological sample comprising at least one nerve feature from the subject with a primary antibody capable of binding phosphorylated alpha-synuclein;
- wherein the primary antibody comprises complementarity determining regions (CDRs) having the following amino acid sequences:
- CDR1 VL
- (SEQ ID NO:
- 2)
- QSVYNNNN,
- CDR2 VL
- (SEQ ID NO:
- 3)
- KASKVAS,
- CDR3 VL
- (SEQ ID NO:
- 4)
- LGGYSGDIYT,
- CDR4 VH
- (SEQ ID NO:
- 6)
- GFTISSYHMS,
- CDR5 VH
- (SEQ ID NO:
- 7)
- ISTSGNI,
- and
- CDR6 VH
- (SEQ ID NO:
- 8)
- ARLGIATGYSF;
- (b) detecting whether the primary antibody capable of binding phosphorylated alpha-synuclein localizes within the nerve feature of the biological sample;
- and
- (c) determining the subject has PD when the primary antibody capable of binding phosphorylated alpha-synuclein localizes within the nerve feature.
27 more elements
A kit comprising:
- (a) a primary antibody capable of binding phosphorylated alpha-synuclein wherein the primary antibody comprises complementarity determining regions (CDRs) having the following amino acid sequences:
- CDR1 VL
- (SEQ ID NO:
- 2)
- QSVYNNNN,
- CDR2 VL
- (SEQ ID NO:
- 3)
- KASKVAS,
- CDR3 VL
- (SEQ ID NO:
- 4)
- LGGYSGDIYT,
- CDR4 VH
- (SEQ ID NO:
- 6)
- GFTISSYHMS,
- CDR5 VH
- (SEQ ID NO:
- 7)
- ISTSGNI,
- and
- CDR6 VH
- (SEQ ID NO:
- 8)
- ARLGIATGYSF;
- and
- (b) a primary antibody capable of binding to a nerve feature.
25 more elements
Dependencies are inferred from the claim text.