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US 12,372,531 B2Granted
Application of click chemistry for signal amplification in IHC and ISH assays
AssigneeVentana Medical Systems, Inc.
InventorsBrian D. Kelly, Nathan W. Polaske
Life of this patent
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Abstract
Applicants have developed an amplification system and methodology for IHC and ISH staining that utilizes “click chemistry” to covalently bind reporter molecules to tissue.
Claims9
A conjugate having Formula (IV):
- wherein
- A is selected from the group consisting of dibenzocyclooctyne, trans-cyclooctene, azide, tetrazine, thiol, 1,3-nitrone, aldehyde, ketone, hydrazine, and hydroxylamine;
- ‘Linker’ is a branched or unbranched, linear or cyclic, substituted or unsubstituted, saturated or unsaturated, group having between 2 and 80 carbon atoms, and optionally having one or more heteroatoms selected from O, N, or S;
- Z is selected from the group consisting of a chromophore, an enzyme, and a hapten;
- “Scaffold” is a polyamine selected from the group consisting of lysine, norspermidine and spermine;
- and
- v is an integer ranging from between 2 and 20.
4 more elements
Dependencies are inferred from the claim text.