Methods for chromatography resin slurry determination

A method for determining a slurry concentration for a slurry of a chromatography resin during column packing, comprising:

• adding a slurry comprising a resin to a first chromatography column;
• setting an automated pump to run at a constant flow rate for a period of time;
• pumping a volume of liquid through the first chromatography column at the flow rate that matches a consolidation rate employed by a second chromatography column to form a consolidated resin in the first chromatography column, wherein the volume of liquid is pumped through the first chromatography column by the automated pump, wherein the automated pump is programed to cycle on and off for a predetermined period of time or for multiple periods of time;
• upon completion of the pumping step, measuring a bed height of the consolidated resin of the first chromatography column;
• determining a slurry concentration from the measured bed height in the first chromatography column;
• and
• packing the second chromatography column with the determined slurry concentration in the first chromatography column, wherein the second chromatography column has a packing factor within 5% of an ideal packing factor.

A method for determining a slurry concentration for a slurry of a chromatography resin during a column packing, comprising:

• providing a slurry comprising a resin;
• mixing the slurry to uniformly distribute the resin;
• adding the slurry to a first chromatography column;
• providing an automated pump system comprising a pump having a controller and, wherein the automated pump system is configured to pump a volume of water through the first chromatography column and the controller is configured to control a flow rate of the volume of water to match a consolidation rate employed by a second chromatography column, wherein the flow of the volume of water will pack the resin in the first chromatography column;
• pumping the volume of water through the first chromatography column at a constant flow rate to form a consolidated resin, wherein the automated syringe pump system is programed to cycle on and off for a predetermined period of time or for multiple periods of time;
• upon completion of the pumping step, measuring a bed height of the consolidated resin of the first chromatography column;
• determining a slurry concentration from the measured bed height of the first chromatography column;
• inputting the determined slurry concentration into the automated pump system;
• and packing the resin in the second chromatography column using the determined slurry concentration in the first chromatography column, wherein the second chromatography column has a packing factor within 5% of an ideal packing factor.