Calibration standard for a device for image-based representation of biological material

A radiation intensity calibration standard for calibrating a device for an image-based representation of biological material, the radiation intensity calibration standard comprising:

• a housing which at least in regions surrounds a luminescent substance in a housing interior, and is made at least partially of a material that is transparent to excitation radiation and to luminescence radiation, and
• an intensity reducer that comprises a partially transparent gray filter arranged on a surface of a window that faces the luminescent substance configured to reduce the radiation intensity of the luminescent substance to a level that matches a radiation intensity emitted by the biological material,
• wherein said housing comprises a point of attachment on an exterior of said housing which is attachable in or on said device for image-based representation of biological material,
• wherein said point of attachment has a thread, which is suitable for being attached repeatedly at a counterpart of the device having a matching contour, and
• wherein said window has a mark for ascertaining a defined distance between the imager and the calibration standard.

A device for image-based representation of biological material, the device comprising:

• an illuminator having a radiation source of electromagnetic excitation radiation,
• a receptacle configured to position the biological material at a first location, wherein the biological material is arranged on a carrier, within a beam path of said electromagnetic excitation radiation,
• at least one imager configured to be in communication with a controller, the controller configured to generate a calibration signal corresponding to a calibration radiation, the at least one imager configured to receive luminescence radiation emitted by the biological material and to generate an image of one or more regions of the biological material that are excited to luminescence, and
• a radiation intensity calibration standard at a second location of the receptacle different from the first location and which is not configured for the biological material, the radiation intensity calibration standard configured to emit the calibration radiation in response to the excitation with the excitation radiation, wherein said radiation intensity calibration standard has a housing that is removably attached to the receptacle, wherein a luminescent substance is disposed inside the housing,
• wherein the radiation intensity calibration standard has an intensity reducer that is a partially transparent gray filter arranged on a surface of a window that faces the luminescent substance and is configured to reduce the radiation intensity of the luminescent substance to a level that matches a radiation intensity emitted by the biological material,
• wherein the luminescent substance arranged in the housing is either pulverulent and comprises a fluorochrome or is an epoxide resin which is excitable to luminescence or a polymer excitable to luminescence, and
• wherein said window has a mark for ascertaining a defined distance between the imager and the calibration standard.