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US 12,371,855 B2Granted
Use of ensiled biomass for increased efficiency of the pretreatment of biomass
AssigneesThe Regents of the University of California, National Technology & Engineering Solutions of Sandia, LLC
InventorsHarsha D. Magurudeniya, Alberto Rodriguez, Nawa Raj Baral, Blake A. Simmons, John M. Gladden
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Abstract
The present invention provides for a method to deconstruct a biomass: the method comprising: (a) ensiling a biomass to produce comprising one or more organic acids, and (b) introducing a solvent to the ensiled biomass to dissolve at least part of solid biomass in the solvent, wherein the solvent is an ionic liquid (IL) or deep eutectic solvent (DES), or mixture thereof, to form a solubilized biomass mixture.
Claims11
A method to deconstruct a biomass:
- the method comprising:
- (a) ensiling a biomass to produce one or more organic acids,
- (b) introducing a solvent to the ensiled biomass to dissolve at least part of solid biomass in the solvent, wherein the solvent is an ionic liquid (IL), to form a solubilized biomass mixture, wherein the solvent introduced is 2.5 to 10% by weight of the ensiled biomass,
- (c) introducing an enzyme to the solubilized biomass mixture such that the enzyme produces a sugar from the solubilized biomass mixture, and
- (d) introducing a genetically modified host cell to the solubilized biomass such that the genetically modified host cell converts the sugar produced from the biomass into a biofuel or chemical compound.
2 more elements
Dependencies are inferred from the claim text.