Recombinant host cells with improved production of L-DOPA, dopamine, S-noroclaurine or derivatives thereof
AssigneeRiver Stone Biotech Aps
InventorsEsben Halkjaer Hansen, Jens Houghton-Larsen
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Abstract
The present invention relates to a recombinant microbial host cell comprising an operative biosynthetic metabolic pathway capable of producing one or more compounds selected from the group consisting of L-dopa, dopamine, (S)-Norcoclaurine and derivatives thereof; said pathway comprising a heterologous L-tyrosine hydroxylase (TyrH) converting L-Tyrosine into L-dopa capable of increasing the cell production of the Compound compared to a reference L-tyrosine hydroxylase having the sequence set forth in SEQ ID NO: 58.
Claims17
A recombinant microbial host cell comprising an operative biosynthetic metabolic pathway capable of producing one or more target compounds selected from the group consisting of L-dopa, dopamine, (S)-Norcoclaurine, and derivatives thereof;
- wherein said pathway comprises one or more heterologous L-tyrosine hydroxylases (TyrH) capable of converting L-Tyrosine into L-dopa and capable of increasing the cell production of the one or more target compounds compared to a reference L-tyrosine hydroxylase having the sequence set forth in SEQ ID NO:
- 58, wherein the one or more heterologous TyrH is a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.