Back to results
US 12,371,713 B2Granted
Method for producing DNA-edited eukaryotic cell, and kit used in the same
AssigneeOsaka University
InventorsTomoji Mashimo, Junji Takeda, Hiroyuki Morisaka, Kazuto Yoshimi
Life of this patent
Estimating the term…
Abstract
A CRISPR-Cas3 system was successfully established in a eukaryotic cell.
Claims5
A method for cleaving endogenous DNA of a eukaryotic cell in the eukaryotic cell, comprising:
- introducing a CRISPR-Cas3 system that can cleave endogenous DNA in a eukaryotic cell, wherein the CRISPR-Cas3 system is a Type I-D CRISPR-Cas3 system, and wherein the CRISPR-Cas3 system includes the following (A) to (C):
- (A) a Cas3 protein with helicase activity, a polynucleotide encoding the protein, or an expression vector containing the polynucleotide,
- (B) Cascade proteins comprising Cas10, Cas5, Cas6, and Cas7, a polynucleotide encoding the Cascade proteins, or an expression vector containing the polynucleotide, and
- (C) a pre-crRNA which targets the endogenous DNA in the eukaryotic cell, a polynucleotide encoding the pre-crRNA, or an expression vector containing the polynucleotide.
1 more element
A method for cleaving endogenous DNA of a nonhuman animal or plant in the nonhuman animal or plant, comprising:
- introducing a CRISPR-Cas3 system that can cleave endogenous DNA in a nonhuman animal or plant, wherein the CRISPR-Cas3 system is a Type I-D CRISPR-Cas3 system, and wherein the CRISPR-Cas3 system includes the following (A) to (C):
- (A) a Cas3 protein with helicase activity, a polynucleotide encoding the protein, or an expression vector containing the polynucleotide,
- (B) Cascade proteins comprising Cas10, Cas5, Cas6, and Cas7, a polynucleotide encoding the Cascade proteins, or an expression vector containing the polynucleotide, and
- (C) a pre-crRNA which targets the endogenous DNA in the nonhuman animal or plant, a polynucleotide encoding the pre-crRNA, or an expression vector containing the polynucleotide.
1 more element
Dependencies are inferred from the claim text.