Back to results
US 12,371,686 B2Granted
Ribulose-phosphate 3-epimerase motif having low side reactivity and enzyme including the same
AssigneeCj Cheiljedang Corporation
InventorsSang Young Yoon, Byung-sam Son, Hyun Kug Cho, Hyun June Park, Seung Hwan Kim, Sungjae Yang, Il Hyang Park, Seong Bo Kim
Life of this patent
Estimating the term…
Abstract
Provided are ribulose-phosphate 3-epimerase, a microorganism and a composition, each including the ribulose-phosphate 3-epimerase, and a method of producing psicose-6-phosphate or psicose using the same.
Claims20
A method of producing psicose-6-phosphate, the method comprising
- the step of bringing fructose-6-phosphate into contact with ribulose-phosphate 3-epimerase, a microorganism expressing the ribulose-phosphate 3-epimerase, or a culture of the microorganism such that the ribulose-phosphate 3-epimerase catalyzes the conversion of fructose-6-phosphate into psicose-6-phosphate,
- wherein the ribulose-phosphate 3-epimerase includes a motif I consisting of an amino acid sequence of SEQ ID NO:
- 1 and a motif III consisting of an amino acid sequence of SEQ ID NO:
- 3, and does not include a motif II consisting of an amino acid sequence of SEQ ID NO:
- 2, and wherein the motifs I and III are present at a substrate and/or a metal ion binding site of the enzyme.
2 more elements
A method of producing psicose, the method comprising steps of
- bringing fructose-6-phosphate into contact with ribulose-phosphate 3-epimerase, a microorganism expressing the ribulose-phosphate 3-epimerase, or a culture of the microorganism such that the ribulose-phosphate 3-epimerase catalyzes the conversion of fructose-6-phosphate into psicose-6-phosphate;
- and
- bringing psicose-6-phosphate produced from the fructose-6-phosphate into contact with psicose-6-phosphate phosphatase, a microorganism expressing the psicose-6-phosphate phosphatase, or a culture of the microorganism,
- wherein the ribulose-phosphate 3-epimerase includes a motif I consisting of an amino acid sequence of SEQ ID NO:
- 1 and a motif III consisting of an amino acid sequence of SEQ ID NO:
- 3, and does not include a motif II consisting of an amino acid sequence of SEQ ID NO:
- 2, and wherein the motifs I and III are present at a substrate and/or a metal ion binding site of the enzyme.
4 more elements
Dependencies are inferred from the claim text.