Process and microorganism for synthesis of adipic acid from carboxylic acids
AssigneeThe Governing Council of the University of Toronto
InventorsAlexander Yakunin, Anna Khusnutdinova, Jeong Chan Joo, Radhakrishnan Mahadevan
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Abstract
A method for biosynthesis of polymer precursors, including, adipic acid, 1,6-hexanediol, 6-hydroxyhexanoic and 6-aminocaproic acids from carboxylic acids is provided. A method for biosynthesis of adipic acid from six-carbon dicarboxylic acids having α, β-enoate reductase activity by treatment with an enzyme is provided. The biocatalytic conversion of aliphatic and hydroxycarboxylic acids to corresponding aldehydes, alcohols, and amines using novel carboxylate reductases, aldehyde reductases, and aminotransferases is described. Also provided are genetically engineered microorganisms for use in the biosynthetic processes.
Claims20
A process for producing a bifunctional aldehyde, as a product or an intermediate, the process comprising enzymatically converting a bifunctional carboxylic acid to a corresponding bifunctional aldehyde using a polypeptide having carboxylate reductase activity, wherein the polypeptide comprises an amino acid sequence at least 90% identical to the amino acid sequence of any one of SEQ ID NOs:
- 35, 36, 37, 38, or 70, wherein in addition to a first carboxyl functional group, the bifunctional carboxylic acid has an oxo-, a hydroxy-, an amino-, or a second carboxyl group as a second functional group, and wherein there are at least three carbons between the first carboxyl functional group and the second functional group.
A process for producing a bifunctional aldehyde, as a product or an intermediate, the process comprising enzymatically converting a bifunctional carboxylic acid to a corresponding bifunctional aldehyde using a polypeptide having carboxylate reductase activity, wherein the polypeptide comprises an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO:
- 35, 36, 37, or 38.
Dependencies are inferred from the claim text.