Bioreactor for RNA in vitro transcription

A bioreactor (1) for RNA in vitro transcription comprising:

• (a) a reaction vessel (2) comprising magnetic particles, DNA templates, a DNA immobilization buffer, DNA magnetic particles and an in vitro transcription (IVT) master mix, said IVT master mix comprising ribonucleoside triphosphates and DNA dependent RNA polymerase,
• wherein the DNA magnetic particles are DNA templates immobilized on the magnetic particles, and
• (b) a magnet unit (3) positioned at the reaction vessel,
• wherein the magnet unit is configured to capture or to introduce a movement of the magnetic particles and the DNA magnetic particles,
• wherein the magnet unit (3) comprises a magnetic ring (31), and wherein the magnetic ring (31) is designed to surround the reaction vessel (2), wherein:
• the magnet unit (3) is a permanent magnet or an electromagnet movable in a longitudinal direction (362) along a longitudinal axis of the reaction vessel (2) and/or a transversal direction (363) towards and apart from the reaction vessel (2);
• the magnet unit (3) is configured to rotate around the longitudinal axis of the reaction vessel (2), and wherein a rotation direction of the magnet unit (3) is switchable during mixing;
• and
• the magnet unit (3) further comprises a first driving means (36) configured to rotate the magnetic ring (31) and a second driving means (37) configured to move the magnetic ring (31) in the vertical direction.