Minimally swellable biocompatible membrane and preparation method thereof

A preparation method of a swellable biocompatible membrane, characterized in that the method comprises the following steps:

• A. synthesis of a copolymer containing a hydrophobic skeleton and hydrophilic moieties;
• A1. dissolving a hydrophobic monomer and a monomer containing a hydrophilic group in ethanol, and removing oxygen with nitrogen;
• A2. adding azodiisobutyronitrile, and placing the obtained solution in an airtight container for reaction;
• and
• A3. separating and purifying to obtain a precipitate, which is the copolymer containing the hydrophobic skeleton and hydrophilic moieties;
• B. introduction of biocompatibility and preparation of a biocompatible membrane solution;
• B1. dissolving the copolymer containing the hydrophobic skeleton and hydrophilic moieties in ethanol;
• and
• B2. adding a biocompatible substance and a crosslinking agent, mixing thoroughly, and then reacting in a water bath to obtain the biocompatible membrane solution;
• and
• C. coating of the biocompatible membrane;
• coating the biocompatible membrane solution on a biosensor directly or after dilution of the biocompatible membrane solution, and drying at room temperature to form a membrane, thus obtaining the swellable biocompatible membrane,
• wherein the separating and purifying operation in A3 is specifically as follows:
• adding water to precipitate the copolymer containing the hydrophobic membrane skeleton and hydrophilic moieties, centrifuging, removing a supernatant, adding ethanol to dissolve the precipitate, and repeating for 2-6 times.