Methods for obtaining antibodies that bind transmembrane proteins and cells that produce the same
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsGang Chen, Ergang Shi, Wen-Yi Lee, David Suh, Glen Farr, Robert Babb
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Abstract
Disclosed are methods for obtaining cells that express antibodies that bind transmembrane proteins, methods for generating antibodies from such cells, antibodies to transmembrane proteins and fragments thereof, and nucleic acids encoding the antibodies. More particularly, the disclosure relates to methods for obtaining antibody-producing cells that express an antibody that binds to a transmembrane protein based on the use of lipid bilayer-membrane scaffold protein complexes to present transmembrane protein antigens to cells.
Claims39
A method for obtaining an antibody-producing cell that expresses an antibody that binds to a transmembrane protein of interest, the method comprising:
- (a) contacting a population of antibody-producing cells with a lipid bilayer-membrane scaffold protein complex comprising the transmembrane protein of interest and with at least one blocking agent, to permit the transmembrane protein of interest to bind to an antibody expressed on the cell surface;
- and
- (b) collecting cells bound to the transmembrane protein of interest.
Dependencies are inferred from the claim text.