Removable biocompatible substrate filter for a reaming and collection device

A method for operating a second stage biocompatible substrate filter comprising:

• configuring biocompatible implant material to retain second stage operative particulate matter;
• combining the second stage biocompatible substrate filter to process first stage operative particulate matter captured from irrigation fluid by a first stage filter, thereby producing a combined product;
• wherein the first stage operative particulate matter includes material of a first size;
• and
• wherein the second stage operative particulate matter includes material of a second size.

A method comprising:

• retaining, via biocompatible implant material disposed within a removable second stage biocompatible filter, second stage operative particulate matter;
• using the removable second stage biocompatible filter to combine operative particulate matter captured from irrigation fluid received via a first stage filter;
• the first stage filter further retaining first stage retained material;
• wherein the first stage retained material and the second stage operative particulate matter are obtained from a reaming device;
• connecting an output of the reaming device to an input of a first stage filter container;
• such that
• a flow of materials suspended in the irrigation fluid is filtered by the first stage filter;
• the first stage retained material is separated from a flow of materials suspended in the irrigation fluid by a filter possessing a first particulate size selectivity that provides a first stage output flow of materials suspended in the irrigation fluid;
• and
• the first stage output flow of materials is further passed through a first stage output;
• connecting an input of a second stage filter container by a second connecting tube to an output of the first stage filter container;
• such that
• the first stage output flow of materials suspended in the irrigation fluid is filtered by the second stage filter;
• the second stage filter retains a second sized particulate matter different than the first stage filter;
• the second stage filter collects a portion of at least one or more of plasma, stem cells, growth factors, and cellular matter, and allows for passing of a portion of the irrigation fluid, as output flow;
• the second stage filter container includes an evacuation port to permit the flow of materials suspended in the irrigation fluid to be evacuated from the second stage filter container in a continuous process;
• the continuous process includes the first stage filter container receiving output from the reaming device and output from the second stage filter simultaneously;
• and
• wherein the removable second stage biocompatible filter is configured to receive at least a portion of the first stage retained material in combination with at least a portion of the second stage retained material to create a combined graft product.