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US 12,370,253 B2Granted
Pre-immunization and immunotherapy
AssigneeAmerican Gene Technologies International Inc.
InventorsCharles David Pauza, Haishan Li, Tyler Lahusen, Jeffrey A. Galvin
Life of this patent
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Abstract
The present invention relates generally to immunization and immunotherapy for the treatment or prevention of HIV. In particular, the methods include in vivo and/or ex vivo enrichment of HIV-specific CD4+ T cells.
Claims10
A method for treating HIV infection in a subject in need thereof comprising:
- (a) ex vivo contacting peripheral blood mononuclear cells (PBMC) isolated from a subject with a stimulatory agent;
- (b) transducing the PBMC ex vivo with a viral delivery system encoding at least one genetic element;
- and
- (c) culturing the transduced PBMC for at least 1 day,
- wherein the at least one genetic element comprises (i) a sequence having at least 90% sequence identity with SEQ ID NO:
- 6, (ii) a sequence having at least 90% sequence identity with SEQ ID NO:
- 7, and (iii) a sequence having at least 90% sequence identity with SEQ ID NO:
- 97, wherein the at least one genetic element does not comprise any small RNA targeting HIV sequences other than Vif and Tat.
5 more elements
Dependencies are inferred from the claim text.