Compositions and methods for obtaining organoids
AssigneeStemcell Technologies Canada Inc.
InventorsCharis-Patricia Segeritz, Ryan Conder, Michael Riedel
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Abstract
A method for obtaining epithelial organoids is provided. In one embodiment, the method comprises culturing one or more epithelial ducts, epithelial duct fragments and/or epithelial stem cells isolated therefrom in contact with an extracellular matrix in the presence of a basal medium, wherein the medium is free of FGF and/or nicotinamide. Organoids obtained by the methods described herein, and uses thereof, are also provided.
Claims18
A method for obtaining an epithelial organoid, the method comprising culturing one or more epithelial ducts, epithelial duct fragments and/or epithelial stem or progenitor cells isolated therefrom in the presence of a basal medium and a wnt agonist, wherein the medium is free from an inhibitor of BMP signaling, and wherein the medium is capable of supporting the organoids for 5 or more passages.
A method for obtaining an epithelial organoid, the method comprising:
- isolating an organ comprising epithelial tissue or a portion thereof;
- cutting and disrupting mechanically the isolated organ or the portion thereof to yield one or more pieces of epithelial tissue;
- digesting enzymatically the one or more pieces of epithelial tissue to yield epithelial ducts and/or duct fragments;
- collecting the digested epithelial ducts and/or duct fragments through a cell strainer;
- plating the collected epithelial ducts and/or duct fragments;
- and
- culturing the plated epithelial ducts and/or duct fragments in the presence of a basal medium and a wnt agonist, wherein the medium is free from an inhibitor of BMP signaling, and wherein the medium is capable of supporting the organoids for 5 or more passages.
Dependencies are inferred from the claim text.