Composition of matter and methods for a fecal-derived sterilized prebiotic and postbiotic

A method for manufacturing a composition for a fecal-derived sterilized prebiotic and postbiotic, the method comprising:

• collecting a pre-donation stool sample from a donor, wherein collecting the pre-donation stool sample from the donor further comprises:
• collecting a serum sample from the donor;
• testing the pre-donation stool sample for presence of infectious disease symptoms, wherein testing the pre-donation stool sample is done within 14 days of collection of the pre-donation stool sample;
• testing the serum sample in response to qualifying the pre-donation stool sample by testing the pre-donation stool sample;
• and
• determining that the donor is healthy based on the testing of the pre-donation stool sample and the serum sample;
• collecting a donation stool sample from the donor, wherein the donation stool sample is frozen;
• sterilizing the donation stool sample while retaining a bacterial plurality of biological macromolecules;
• blending the donation stool sample with a liquid to create a fecal-derived composition;
• identifying a PH of the fecal-derived composition;
• increasing the PH of the fecal-derived composition to value of at least 7.2 prior to lyophilization to maintain a plurality of short chain fatty acids during the lyophilization;
• stabilizing the fecal-derived composition by the lyophilization, wherein the lyophilization results in a freeze-dried, sterilized fecal-derived composition with fiber and particulate matter included;
• monitoring, by a moisture probe, a moisture content of the freeze-dried, sterilized fecal-derived composition;
• confirming that the moisture content of the freeze-dried, sterilized fecal-derived composition is less than 8%;
• and
• verifying that the composition includes a respective ratio by weight of acetic acid:propionic acid:butyric acid of 60±10:20±10:20±10.