Systems and methods for live projection imaging for fluorescence microscopy
AssigneeBoard of Regents, The University of Texas System
InventorReto Fiolka
Life of this patent
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Abstract
Implementations discussed and claimed herein provide systems and methods live projection imaging for fluorescence microscopy. In one implementation, a 3D view of a sample, such as cells, is generated for direct viewing. A projection of a volume is generated that is optically sheared into a single camera frame in light-sheet fluorescence microscopy. Optical shearing is synchronized with acquisition of a volume, where volumetric information may be directly viewed in a single acquisition to evaluate cellular 3D morphologies and dynamics.
Claims20
A method for imaging a sample using a microscopy system, the method comprising:
- scanning the sample along a scanning direction while illuminating the sample with light generated by at least one light source along an illuminating direction;
- shearing fluorescence light emitted by the sample in response to illumination of the sample, the fluorescence light sheared optically using a shearing unit to form a plurality of sheared volume image planes which create a sheared volume;
- and
- projecting an individual sheared volume image plane of the plurality of sheared volume image planes onto a single camera frame of an imager to permit viewing in real time using the shearing unit.
Dependencies are inferred from the claim text.