Identification of platelet activating antibodies
AssigneeRetham Technologies, Inc.
InventorsAnand Padmanabhan, Curtis G. Jones
Life of this patent
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Abstract
Described is a method for identifying pathogenic platelet-activating antibodies in a subject's blood and particularly antibodies implicated in heparin-induced thrombocytopenia (HIT) which comprises the preparation of a platelet releasate from a normal subject's platelets, the combination of the platelet release with a normal subject's platelets, a test subject's blood sample, and analyzing the sample for platelet activation.
Claims26
A method for identifying platelet-activating heparin-induced thrombocytopenia (HIT) antibodies in a blood sample from a subject suspected of having platelet-activating HIT antibodies, the method comprising:
- obtaining a blood sample comprising serum, plasma, or fractionated blood from a subject suspected of having platelet-activating HIT antibodies;
- obtaining platelets from one or more normal subjects;
- obtaining a platelet releasate from the platelets from one or more normal subjects by activating the platelets from the one or more normal subjects with a platelet activator and collecting material released from the activated platelets, forming a platelet releasate;
- combining the platelet releasate from the one or more normal subjects, the platelets from the one or more normal subjects, and the sample from the subject suspected of having platelet-activating antibodies, forming a combined sample, and incubating the combined sample for about 5 min. to about 4 hours;
- and
- measuring platelet activation in the combined sample;
- wherein platelet activation in the combined sample is indicative of the presence of platelet-activating HIT antibodies in the blood sample from the subject suspected of having platelet-activating antibodies.
A method for identifying platelet-activating heparin-induced thrombocytopenia (HIT) antibodies in a blood sample from a subject suspected of having platelet-activating HIT antibodies, the method comprising:
- obtaining a blood sample comprising serum, plasma, or fractionated blood from a subject suspected of having platelet-activating HIT antibodies;
- obtaining platelets from one or more normal subjects;
- obtaining a platelet releasate from the platelets from one or more normal subjects by activating the platelets from one or more normal subjects with a platelet activator, collecting material released from the activated platelets, and contacting the material released from the activated platelets with an anion-exchanger forming a platelet releasate;
- combining the platelet releasate with platelets from one or more normal subjects, forming a first combined sample and incubating the first combined sample for about 5 min. to about 1 hour;
- combining the subject sample with the first combined sample, forming a second combined sample, and incubating the second combined sample for 5 min. to about 5 hours;
- measuring platelet activation in the second combined sample;
- wherein platelet activation in the second combined sample is indicative of the presence of platelet-activating HIT antibodies in the blood sample from the subject suspected of having platelet-activating antibodies.
Dependencies are inferred from the claim text.