Adapter-based retroviral vector system for the selective transduction of target cells

A combination of reagents configured for delivery of a gene or protein to a target cell, consisting of:

• (i) a tagged polypeptide that comprises:
• (a) an antigen binding moiety (ABM), which binds specifically to said antigen on the target cell;
• (b) a label moiety (LaM), which is a hapten, and
• (c) a linker moiety (LiM), which conjugates the ABM to the LaM such that the LaM and LiM together form a linker/label epitope (LLE);
• and
• (ii) a pseudotyped Paramyxoviridae viral vector that contains said gene or protein for delivery to the target cell;
• wherein the pseudotyped Paramyxoviridae viral vector is a lentiviral or gammaretroviral vector that comprises:
• (a) a Paramyxoviridae attachment protein that has been genetically modified to remove constitutive cell binding activity, fused at or near the C-terminal of its ectodomain to a single chain antibody that comprises a binding domain that specifically binds to the LLE on the tagged polypeptide, and
• (b) a Paramyxoviridae fusion protein that has fusion activity;
• wherein said attachment protein is a Morbillivirus hemagglutinin (H) or a Henipavirus glycoprotein (G);
• and
• wherein said fusion (F) protein is from the same Paramyxoviridae genus as the attachment protein;
• wherein contacting the target cell with said viral vector in the presence of said tagged polypeptide results in delivery of said gene or protein into the target cell.