Disease resistant squash plants
AssigneeEnza Zaden Beheer B.V.
InventorsBart Kaij, Yusuf Sen, Quy Dung Dinh
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Abstract
The present disclosure relates to squash plants that are resistant to the plant disease downy mildew caused by the pathogen Pseudoperonospora cubensis and/or powdery mildew caused by the pathogen Erysiphe cichoracearum. Specifically, the present disclosure relates to squash plants that are resistant to mildew disease including a mutated homoserine kinase gene encoding a mutated homoserine kinase protein. The present disclosure further relates to seeds, tissues, cells, or plant parts of the present squash plants, and to methods for identifying mildew resistant squash plants.
Claims19
A downy mildew resistant squash plant, wherein said squash plant comprises in its genome a homoserine kinase gene expressing a mutated homoserine kinase protein, wherein the mutation of the homoserine kinase protein comprises an amino acid substitution of the amino acid alanine (A) to the amino acid threonine (T) at position 303 of a homoserine kinase protein of which the amino acid sequence is shown in SEQ ID No. 1.
A mutated homoserine kinase protein, wherein the mutation comprises an amino acid substitution of the amino acid alanine (A) to the amino acid threonine (T) at position 303 of a homoserine kinase protein of which the amino acid sequence is shown in SEQ ID No. 1.
A method of producing a squash plant that is resistant to a plant pathogen that causes downy mildew, the method comprising introducing a mutation into a homoserine kinase gene that encodes a protein having the sequence of SEQ ID NO:
- 1 in a plant that is susceptible to the plant pathogen that causes downy mildew, the mutation resulting in an A303T substitution in SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.