Back to results
US 12,365,902 B2Granted
Compositions and methods of generating novel amiRNA
AssigneeMirimus, Inc.
InventorsGiuseppe Militello, Christof Fellmann, Johannes Zuber
Life of this patent
Estimating the term…
Abstract
Provided herein are Compositions and Methods of Generating Novel amiRNA's. Provided herein are methods for producing or generating one or more amiRNAs; also provided are constructs and compositions useful in the methods. The methods and constructs provided in this disclosure are highly efficient methods for production of a new generation of amiRNAs.
Claims9
A method of generating amiRNAs and modifying a selected primary miRNA, comprising:
- Applying a base modifying step to produce a single stranded region of at least 9 nucleotides and a GU dinucleotide in position −13 and −14, respectively from a Drosha cleavage site to form a base modified construct;
- Applying a basal stem step to produce a double stranded RNA with a CHC motif and a bulge in position +8 from the Drosha cleavage site to form a base stem modified construct;
- Applying a loop modifying step to substitute the endogenous loop region of the primary miRNA with the loop of a miRE, or another loop with same number of nucleotides and the GUG motif in the same position, to form a loop modified construct;
- and
- Operably linking the base modified construct, the basal stem modified construct, and the loop modified construct with the primary miRNA into an amiRNA construct.
2 more elements
Dependencies are inferred from the claim text.