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US 12,365,891 B2Granted
Methods and compositions for nucleic acid isolation
AssigneeCepheid
InventorsVera Baraznenok, Alex I. Kutyavin, Oliver Z. Nanassy, Dmitri Sergueev, Alexander A. Gall
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Abstract
Methods and compositions for isolation of nucleic acids from biological samples are provided.
Claims19
A method for isolation of a nucleic acid from a sample comprising a nucleic acid, comprising:
- (a) contacting a sample comprising a nucleic acid with an aqueous composition comprising a polysaccharide comprising one or more repeating units having the structure of Formula II:
- or a stereoisomer, a salt, a tautomer, or a combination thereof, wherein:
- R2 and R3 are independently H, substituted C1-C8 alkyl, substituted C3-C8 cycloalkyl, substituted C3-C8 heterocycloalkyl, or substituted C2-C20 heteroalkyl;
- and
- (b) concentrating the nucleic acid on a solid support thereby isolating the nucleic acid, wherein the method is performed in a cartridge.
2 more elements
A method for isolation of a nucleic acid from a sample comprising a nucleic acid, comprising:
- (a) contacting a sample comprising a nucleic acid with an aqueous composition comprising a polysaccharide comprising one or more repeating units having the structure of Formula II:
- or a stereoisomer, a salt, a tautomer, or a combination thereof, wherein:
- R2 and R3 are independently H, substituted C1-C8 alkyl, substituted C3-C8 cycloalkyl, substituted C3-C8 heterocycloalkyl, or substituted C2-C20 heteroalkyl;
- and
- (b) concentrating the nucleic acid on a solid support thereby isolating the nucleic acid, further comprising contacting the sample with a lysis buffer prior to contacting the sample with the aqueous composition.
2 more elements
A method for detecting a nucleic acid in a sample, comprising:
- (a) contacting the sample with an aqueous composition comprising a polysaccharide comprising one or more repeating units having the structure of Formula II:
- or a stereoisomer, a salt, a tautomer, or a combination thereof, wherein:
- R2 and R3 are independently H, substituted C1-C8 alkyl, substituted C3-C8 cycloalkyl, substituted C3-C8 heterocycloalkyl, or substituted C2-C20 heteroalkyl;
- (b) concentrating the nucleic acid;
- and
- (c) detecting the nucleic acid,
- wherein detecting the nucleic acid comprises amplifying the nucleic acid by polymerase chain reaction.
4 more elements
Dependencies are inferred from the claim text.