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US 12,365,889 B2Granted
Tunable nanoscale cages from self-assembling DNA and protein building blocks
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsNicholas Stephanopoulos, Yang Xu
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Abstract
Described herein are polyhedral, three-dimensional tunable nanocages assembled with a multimeric protein covalently linked to a polynucleotide handle and a DNA origami base assembly including sequences complementary to the polynucleotide handles, wherein the polynucleotide handle and the complementary sequences hybridize to for double-stranded DNA helices.
Claims6
A polyhedral nanocage comprising:
- a multimeric protein, wherein each monomer of the multimeric protein is covalently linked to a polynucleotide handle;
- and
- a DNA assembly of at least 4 oligonucleotides comprising a polynucleotide arm complementary to the polynucleotide handle, wherein the polynucleotide handle and the polynucleotide arm are hybridized to form a double stranded DNA helix linking the multimeric protein to the DNA assembly;
- wherein polynucleotide handle is at least 15 base pairs long;
- wherein the polynucleotide handle is covalently linked to a solvent-exposed cysteine residue by a disulfide linkage;
- and
- wherein the multimeric protein comprises a surface 4-azidophenylalanine residue.
4 more elements
Dependencies are inferred from the claim text.