Production of riboflavin
AssigneeDsm IP Assets B.V.
InventorsChristopher Mark Farrell, Sébastien Eric Potot, Zoltán Prágai, Michael Patrick Anaya
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Abstract
The present invention is related to an improved process for production of vitamin B2 using a genetically engineered host cell comprising a heterologous enzyme with pyridoxal phosphatase activity. Using said modified host cell, the yield of riboflavin production could be increased by at least about 5%.
Claims12
A riboflavin-producing Bacillus subtilis host cell expressing a polynucleotide encoding a heterologous enzyme with pyridoxal phosphatase activity, wherein the heterologous enzyme comprises a polypeptide with at least 95% sequence identity to the polypeptide of SEQ ID NO:2, and wherein the host cell comprises a mutation in an endogenous ribC gene that disrupts the expression of the endogenous ribC gene.
A process for production of riboflavin, comprising
- (a) incubating a riboflavin-producing microbial host cell in an aqueous medium under conditions that allow the production of riboflavin from a given substrate;
- and
- (b) isolating the riboflavin from the cultivation medium,
- wherein the riboflavin-producing host cell expresses a polynucleotide encoding a heterologous enzyme with pyridoxal phosphatase activity, wherein the heterologous enzyme comprises a polypeptide with at least 95% sequence identity to the polypeptide of SEQ ID NO:2.
Dependencies are inferred from the claim text.