Compositions and methods for t-cell receptor gene assembly

A composition comprising (a) a plurality of nucleic acid molecules, wherein each nucleic acid molecule of the plurality of nucleic acid molecules comprises (i) a unique anti-connector sequence, (ii) a sequence from a T-cell receptor (TCR) V gene and (iii) does not comprise a complementarity determining region 3 (CDR3) sequence, wherein a first nucleic acid molecule of the plurality comprises a first anti-connector sequence and a second nucleic acid molecule of the plurality comprises a second anti-connector sequence, wherein the first anti-connector sequence is a different nucleic acid sequence from the second anti-connector sequence, wherein the first anti-connector sequence and the second anti-connector sequence encode a same amino acid sequence, and wherein the same amino acid is encoded by a sequence conserved in the TCR V gene adjacent to the sequence encoding the CDR3 in a rearranged gene, and wherein the sequence from a TCR V gene of the first nucleic acid molecule and the second nucleic acid molecule are from different TCR V genes;

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• (b) a first plurality of nucleic acid molecules, wherein each nucleic acid molecule of the first plurality of nucleic acid molecules comprises a sequence encoding a CDR3 of a different TCR chain, wherein each nucleic acid molecule of the first plurality of nucleic acid molecules comprises a unique connector sequence, which unique connector sequence is capable of specifically linking to a unique anti-connector sequence of a nucleic acid molecule of the plurality of nucleic acid molecules of (a) that comprises a sequence from a single given TCR V gene, and wherein the plurality of nucleic acid molecules of (a) and the first plurality of nucleic acid molecules of (b) are in a same compartment.