Epitope tags recognized by specific binders
AssigneeNANOTAG BIOTECHNOLOGIES GmbH
InventorsSteffen Frey, Hansjoerg Goetzke, Davilla Luis Felipe Opazo, Paal Erik Gustav Stenmark, Carranza Markel Martinez
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Abstract
The present invention provides peptides useful as epitope tags, which may be fused to a polypeptide of interest, as well as antibodies that specifically bind to these peptides. The peptides and/or antibodies can be used for detecting, immobilizing, isolating or purifying a molecule that is conjugated to such a peptide and/or antibody.
Claims13
A single domain antibody that is capable of binding a peptide having the sequence SRLEEELRRRLTE (SEQ ID NO:
- 04), wherein the antibody comprises CDR1, CDR2, and CDR3 sequences selected from the group consisting of:
- (a) a CDR1 sequence having the sequence of GVTISALNAMAMG (SEQ ID NO:
- 115), a CDR2 sequence having the sequence of AVSERGNAM (SEQ ID NO:
- 116), and a CDR3 sequence having the sequence of LEDRVDSFHDY (SEQ ID NO:
- 117);
- and
- (b) a CDR1 sequence having the sequence of GVTISALNAMAMG (SEQ ID NO:
- 115), a CDR2 sequence having the sequence of AVSSRGNAM (SEQ ID NO:
- 119), and a CDR3 sequence having the sequence of LEDRVDSFHDY (SEQ ID NO:
- 117).
A single domain antibody comprising the VHH sequence selected from the group consisting of:
- (a)
- (SEQ ID NO:
- 133)
- EVQLQESGGGLVQPGGSLRLSCTASGVTISALNAMAMGWYRQAPGERRV
- MVAAVSERGNAMYRESVQGRFTVTRDFTNKMVSLQMDNLKPEDTAVYYC
- HVLEDRVDSFHDYWGQGTQVTVSS;
- and
- (b)
- (SEQ ID NO:
- 134)
- EVQLQESGGGLVQPGGSLRLSCTASGVTISALNAMAMGWYRQAPGEERV
- MVAAVSSRGNAMYRESVQGRFTVTRDFTNKMVSLQMDNLKPEDTAVYYC
- HVLEDRVDSFHDYWGQGTQVTVSS.
Dependencies are inferred from the claim text.