Back to results
US 12,364,978 B2Granted
Anionic exchange-hydrophobic mixed mode chromatography resins
AssigneeBio-Rad Laboratories, Inc.
InventorsChristopher Belisle, Xuemei He, Hong Chen, Yueping Xu, Jiali Liao
Life of this patent
Estimating the term…
Abstract
Chromatography resins having anionic exchange-hydrophobic mixed mode ligands and methods of using such resins are provided.
Claims6
A chromatography resin having the following formula:
- or an anionic salt thereof,
- wherein:
- X is a spacer;
- R2 is C2 to C6 alkyl or C4 to C6 cycloalkyl;
- L is NR4, O, or S;
- n=1 or 2;
- and
- Ar is a 6-10 membered ring and:
- if Ar is aryl, the aryl is optionally substituted with up to five C1 to C3 unsubstituted alkyl, C3 to C6 branched alkyl, unsubstituted aryl, or fluorine groups;
- or
- if Ar is heteroaryl, the heteroaryl is optionally substituted with up to four unsubstituted alkyl groups.
8 more elements
A chromatography resin prepared by reacting a ligand with a chromatography support comprising an aldehyde group, epoxide group, or an azalactone group, wherein the ligand is selected from the group consisting of:
- bis(2-phenoxyethyl)amine;
- bis[2-(2-phenoxyethoxy)ethyl]amine;
- bis[2-(3,5-xylyloxy)ethyl]amine;
- bis[2-(4-biphenylyloxy)ethyl]amine;
- bis[2-(p-tolyloxy)ethyl]amine;
- bis[2-(o-tolyloxy)ethyl]amine;
- bis[2-(m-tolyloxy)ethyl]amine;
- bis[2-(p-ethylphenoxy)ethyl]amine;
- bis[2-(p-cumenyloxy)ethyl]amine;
- bis{2-[p-(tert-butyl)phenoxy]ethyl}amine;
- bis[2-(1-naphthyloxy)ethyl]amine;
- bis[2-(p-fluorophenoxy)ethyl]amine;
- bis[2-(2,5-difluorophenoxy)ethyl]amine;
- bis[2-(m-fluorophenoxy)ethyl]amine;
- bis[2-(p-cumenyloxy)ethyl]amine;
- bis[2-(3,4-difluorophenoxy)ethyl]amine;
- bis[2-(3,4,5-trifluorophenoxy)ethyl]amine;
- bis[2-(2,3,4,5,6-pentafluorophenoxy)ethyl]amine;
- bis[2-(2,6-dimethyl-4-pyridyloxy)ethyl]amine;
- bis[2-(4-pyridyloxy)ethyl]amine;
- bis[2-(3-pyridyloxy)ethyl]amine;
- bis(3-phenoxycyclobutyl)amine;
- bis(3-phenoxycyclopentyl)amine;
- and
- bis(4-phenoxycyclohexyl)amine.
22 more elements
Dependencies are inferred from the claim text.